Overview
- Peptide (C)SVAVAKAKPKFSIS, corresponding to amino acid residues 372-385 of rat Kir6.2 (Accession P70673). Intracellular, C-terminus.
Western blot analysis of rat heart membranes (lanes 1 and 3) and mouse heart lysate (lanes 2 and 4):1-2. Guinea pig Anti-Kir6.2 Antibody (#APC-020-GP), (1:500).
3-4. Guinea pig Anti-Kir6.2 Antibody, preincubated with Kir6.2 Blocking Peptide (#BLP-PC020).
Western blot analysis of human SHSY-5Y neuroblastoma cell lysate:1. Guinea pig Anti-Kir6.2 Antibody (#APC-020-GP), (1:200).
2. Guinea pig Anti-Kir6.2 Antibody, preincubated with Kir6.2 Blocking Peptide (#BLP-PC020).
Expression of Kir6.2 in mouse hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Guinea pig Anti-Kir6.2 Antibody (#APC-020-GP), (1:300), followed by goat-anti-guinea pig-Cy3 antibody (red staining). Kir6.2 staining appears in the hilus region in neuronal outlines (arrows). Nuclei are stained with DAPI (blue).
- Babenko, A.P. et al. (1998) Annu. Rev. Physiol. 60, 667.
- Huopio, H. et al. (2002) Am. J. Physiol. 283, 207.
Kir6.2 is a member of the inward rectifier K+ channels (Kir channels), a large family of voltage-independent K+ channels largely involved in stabilization of the membrane resting potential and in K+ transport across membranes. Kir channels can be modulated by a variety of intracellular agents such as protons, GTP-binding proteins and adenine nucleotides.
The ATP-sensitive channel (KATP) is especially important since it couples cellular metabolism (intracellular ATP levels) with cell excitability. KATP channels have been described in pancreatic b-cells, neurons, heart, skeletal and smooth muscle.
The KATP channel is composed of a Kir6.2 or Kir6.1 subunit and a sulphonylurea receptor (SUR) subunit.
The pancreatic KATP channel for example, is composed of a complex of Kir6.2 and SUR1 subunits, while the cardiac KATP channel is composed of Kir6.2 and SUR2A complexes.
Impaired b-cell KATP channel function due to mutations in either Kir6.2 or SUR1 subunits has been linked to the recessive autosomal disorder called persistent hyperinsulinemic hypoglycemia of infancy (PHHI). In addition, a Kir6.2 variant has recently been linked to an increased risk of developing type-2 diabetes.
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Kir6.2. Guinea pig Anti-Kir6.2 Antibody (#APC-020-GP, formerly AGP-067) raised in guinea pig can be used in western blot and immunohistochemistry applications. It has been designed to recognize Kir6.2 from human, rat and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-Kir6.2 Antibody (#APC-020) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.
Multiplex staining of Kir6.2 and BDNF in mouse hippocampus.Immunohistochemical staining of immersion-fixed,free floating mouse brain frozen sections using Guinea pig Anti-Kir6.2 Antibody (#APC-020-GP), (1:300) and Anti-BDNF Antibody (#ANT-010), (1:300). A. Kir6.2 staining (red) appears in the dentate gyrus granule layer (G) and in hilar interneurons (arrows). B. BDNF staining (green) in the same section appears in the dentate gyrus granule layer (G) and in hilar interneurons (arrows). C. Merge of the two images reveals colocalization of Kir6.2 and BDNF in hippocampal interneurons.
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