Overview
- Peptide (C)GSWHPRSYAAYALK, corresponding to amino acid residues 292-305 of human KISS1R (Accession Q969F8). Extracellular, 3rd loop.
Cell surface detection of KISS1R by direct flow cytometry in live intact human THP-1 monocytic leukemia cell line:___ Cells.
___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
___ Cells + Anti-KISS1 Receptor (GPR54) (extracellular)-FITC Antibody (#AKR-001-F), (5µg).
Cell surface detection of KISS1R by direct flow cytometry in live intact mouse J774 macrophage cell line:___ Cells.
___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
___ Cells + Anti-KISS1 Receptor (GPR54) (extracellular)-FITC Antibody (#AKR-001-F), (5µg).
Cell surface detection of KISS1R by direct flow cytometry in live intact human Jurkat T-cell leukemia cell line:___ Cells.
___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
___ Cells + Anti-KISS1 Receptor (GPR54) (extracellular)-FITC Antibody (#AKR-001-F), (5µg).
The kisspeptin receptor (KISS1R, GPCR54) is a member of the G-protein coupled receptor superfamily. KISS1R natural ligands are peptides called kisspeptins which are encoded by the Kiss1 gene. The Kiss1 gene was originally discovered by Hershey scientists who named it after their famous chocolate, the “Kiss”.
The kisspeptins, acting through KISS1R, were found to be potent inhibitors of metastasis in a variety of cancer cell types including thyroid and pancreatic cancer cells. KISS1R and kisspeptins are involved in the direct regulation of the reproductive neuroendocrine axis.
KISS1R is widely distributed throughout the brain, where it is most highly expressed in the hypothalamic and amygdaloid nuclei. High expression was also found in the pancreas and placenta. Lower-level expression was found in a variety of other peripheral tissues including peripheral blood leukocytes, kidney, lung, testes, spleen, thymus, adrenal glands and lymph nodes.1-3
