Name: Anti-KV1.3 (KCNA3) (extracellular) Antibody
Brand: Alomone Labs
SKU: APC-101

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Overview

Cat #: APC-101

Alternative Name Potassium voltage-gated channel subfamily A member 3

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide KDYPASTSQDSFEA(C), corresponding to amino acid residues 263-276 of human K_V1.3 (Accession P22001). Extracellular loop between domains S1 and S2.

Accession (Uniprot) Number P22001

Gene ID 3738

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat, mouse - 12/14 amino acid residues identical.

RRID AB_2040149.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ic, if, ifc, ih, ip, lci, wb

Western blot

Rat brain membranes and human Jurkat T lymphocytes (1:200-1:500).
Western blot analysis of rat brain membranes:
1. Anti-K_V1.3 (KCNA3) (extracellular) Antibody (#APC-101), (1:500).
2. Anti-K_V1.3 (KCNA3) (extracellular) Antibody, preincubated with Kv1.3/KCNA3 (extracellular) Blocking Peptide (#BLP-PC101).
Western blot analysis of human Jurkat T cells:
1. Anti-K_V1.3 (KCNA3) (extracellular) Antibody (#APC-101), (1:200)
2. Anti-K_V1.3 (KCNA3) (extracellular) Antibody, preincubated with Kv1.3/KCNA3 (extracellular) Blocking Peptide (#BLP-PC101).

Immunoprecipitation

Human lung carcinoma cell line A549 isolated nuclei (Jang, S.H. et al. (2015) J. Biol. Chem. 290, 12547.).

Immunohistochemistry

Mouse retina (1:200) (Cangiano, L. et al. (2007) PLoS ONE 2, 1327.).

Immunocytochemistry

Human B-cell lymphoma cells (Raji cells), (1:100) (Kawano, T. et al. (2009) Biol. Pharm. Bull. 32, 345.).

Indirect flow cytometry

0.5-5 μg antibody / 1x10⁶cells.

Human T cells (Beeton, C. et al. (2006) Proc. Natl. Acad. Sci. U.S.A. 103, 17414.).
The blocking peptide is not suitable for this application.

Live cell imaging / Immunocytochemistry

HEK 293 transfected cells (1:50).

Scientific background

K_V1.3 belongs to the Shaker family of voltage-dependent K⁺ channels. The channel, encoded by KCNA3, is widely expressed in the brain, lung and osteoclasts and in several cell populations of hematopoietic origin. The prominence of K_V1.3 channels in these cells (particularly in T lymphocytes) directed much research attention. It was found that K_V1.3 is the main channel responsible for maintaining the resting potential in quiescent cells and regulating the Ca²⁺ signaling that is indispensable for normal T lymphocyte activation.^1,2 Based on the central role of K_V1.3 in regulating the initiation of an immune response, the channel has been recognized as a potential target for immunosuppressant drugs.¹ The central role of K_V1.3 in immune system cells created a real need for a specific antibody that would be able to work in flow cytometry applications.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

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Anti-K_V1.3 (KCNA3) (extracellular) Antibody

Overview

Application key:

Species reactivity key:

Specifications

Citations

Applications

Scientific Background

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