Overview
- Peptide (C)GHSRLLNDTSAPHLE, corresponding to amino acid residues 348 - 362 of rat KV1.4 (Accession P15385). 1st extracellular loop.
Western blot analysis of rat brain lysate:1. Anti-KV1.4 (extracellular) Antibody (#APC-167), (1:400).
2. Anti-KV1.4 (extracellular) Antibody, preincubated with Kv1.4 (extracellular) Blocking Peptide (#BLP-PC167).
Western blot analysis of mouse brain lysate:1. Anti-KV1.4 (extracellular) Antibody (#APC-167), (1:200).
2. Anti-KV1.4 (extracellular) Antibody, preincubated with Kv1.4 (extracellular) Blocking Peptide (#BLP-PC167).
Expression of KV1.4 in rat parietal cortexImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-KV1.4 (extracellular) Antibody (#APC-167), (1:400), followed by donkey-anti-rabbit-biotin and streptavidin-Cy3. KV1.4 staining (red) appears in several neuronal-outlined cells (arrows). Nuclei were stained with DAPI (blue).
Expression of KV1.4 in live intact rat PC12 cellsCell surface detection of KV1.4 in live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-KV1.4 (extracellular) Antibody (#APC-167), (1:25), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (red). B. Live view of the cells. C. Merge of A and B.
- Stuhmer, W. et al. (1989) EMBO J. 8, 3235.
- Gutman, G.A. et al. (2005) Pharmacol. Rev. 57, 473.
- Bogin, O. (2006) Modulator 21, 28.
KV1.4 is a mammalian voltage-dependent K+ channel, homologous to the Drosophila Shaker K+ channel. KV1.4 was first cloned from rat brain.1 Eight Shaker-related genes exist in mammals constituting the KV1 subfamily of the large KV channel family of genes.2
A functional KV1 channel is either a membrane spanning homotetramer or heterotetramer, which is composed of members of the same subfamily. In addition, several auxiliary subunits and intracellular proteins might interact with the channel and affect its function.
The structure of KV1.4 channel is similar to all KV channels and includes six membrane spanning helices creating a voltage sensor domain and a pore domain.2
The channel is expressed in neurons and cardiac and skeletal muscle tissue as well as in the pancreas.2 The functional channel is considered transient (A-type) current and shows pronounced inactivation. Therefore, this channel activity influences the membrane potential and excitability of neurons and muscle.
KV1.4 channels are sensitive to high doses of TEA (>100 mM) and low doses of 4-AP (0.013 mM), the “classical” non-selective potassium channel blockers.
Most venomous peptide toxins that affect other KV channels do not inhibit KV1.4. However, the sea anemone toxin Stichodactyla Toxin (ShK), which is more potent towards KV1.1 and KV1.3, is still a potent inhibitor of KV1.4 channels.3
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat KV1.4. Anti-KV1.4 (extracellular) Antibody (#APC-167) can be used in western blot, immunohistochemistry and live cell imaging applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the channel in living cells. It has been designed to recognize KV1.4 from human, rat and mouse samples.
Applications
Citations
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Vivekananda, U. et al. (2017) PNAS 114, 2395.
