Name: Anti-KVβ1 Antibody
Brand: Alomone Labs
SKU: APC-127

Overview

Cat #: APC-127

Alternative Name Voltage-gated potassium channel subunit beta-1, K_V beta 1, K_Vb1, KCNAB1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)TPQHHISLKESTAK, corresponding to amino acid residues 54-67 of rat KCNAB1 (Accession P63144). N-terminus.

Accession (Uniprot) Number P63144

Gene ID 29737

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, human - identical.

RRID AB_2341032.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Specificity This antibody will recognize the "canonical" splice isoform of human K_Vβ1 (also known as K_Vβ1.3). It will not recognize splice variants K_Vβ1.1 and K_Vβ1.2. For more information see human K_Vβ1 entry Q14722.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: wb

May also work in: ic*, ifc*, ih*, ip*

Western blot

Western blot analysis of human SH-SY5Y neuroblastoma cell lysate (lanes 1 and 4), mouse brain lysate (lanes 2 and 5) and rat brain lysate (lanes 3 and 6):
1-3. Anti-K_Vβ1 Antibody (#APC-127), (1:200).
4-6. Anti-K_Vβ1 Antibody, preincubated with Kvβ1 Blocking Peptide (#BLP-PC127).

References

Hille, B. (1992) Sinauer, Sunderland, MA.
Zagotta, W.N. et al. (1990) Science 250, 56.
Pongs, O. et al. (1999) Ann. N. Y. Acad. Sci. 868, 344.
McCormack, K. et al. (1995) FEBS Lett. 370, 32.

Scientific background

Voltage-gated K⁺ channels represent a structurally and functionally diverse group of membrane proteins. These channels establish the resting membrane potential and modulate the frequency and duration of action potentials in nerve and muscle¹. The duration of the open states of K⁺ channels is determined by the rates of deactivation and inactivation. K_V channels are hetero-oligomeric complexes consisting of two different types of subunits: membrane-bound, pore-forming α subunits and the smaller β subunits. The β subunits appear to be peripheral proteins tightly associated with the cytoplasmic side of the α subunits².

The inactivation gate can be formed by an N-terminal segment of an auxiliary β-subunit, as in the complex of K_Vβ1 and K_Vα family channels³. There are three mammalian K_Vβ genes: K_Vβ1, K_Vβ2 and K_Vβ3. Additional variability in the K_Vβ1 family results from alternative splicing in the amino terminal region, thus yielding the K_Vβ1.1, K_Vβ1.2, and K_Vβ1.3 subunits⁴.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat K_Vβ1. Anti-K_Vβ1 Antibody (#APC-127) can be used in western blot analysis. This antibody will recognize the splice isoform of human K_Vβ1 (also known as K_Vβ1.1). It won’t recognize splice variants K_Vβ1.2 and K_Vβ1.3. For more information, see human K_Vβ1 entry Q14722 and Q14722-2. It has been designed to recognize K_Vβ1 from rat, mouse and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Kvβ1 Blocking Peptide (#BLP-PC127) is the original antigen used for immunization during Anti-K_Vβ1 Antibody (#APC-127) generation. The blocking peptide binds and ‘blocks’ Anti-Kvβ1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Kvβ1 Blocking Peptide (#BLP-PC127)

Shipping and Ordering Information

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Anti-K_Vβ1 Antibody