Overview
Alternative Name KV10.2, Ether-a-go-go potassium channel 2, hEAG2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, r
Immunogen
- Peptide (C)EDPKGSDSENSVTKNPLRK, corresponding to amino acid residues 842-860 of rat KCNH5 (Accession Q9EPI9). Intracellular, C-terminus.
Accession (Uniprot) Number Q9EPI9
Gene ID 171146
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human - 18/19 amino acid residues identical.
RRID AB_2039935.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Isotype Rabbit IgG.
Specificity The antibody is specific for KV10.2 and will not cross-react with KV10.1.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Reconstitution 0.2 ml double distilled water (DDW).
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
- Rat brain membranes (1:200).
Western blot analysis of rat brain membranes:1. Anti-KCNH5 (EAG-2) Antibody (#APC-053), (1:200).
2. Anti-KCNH5 (EAG-2) Antibody, preincubated with KCNH5/EAG-2 Blocking Peptide (#BLP-PC053).
Immunohistochemistry
- Rat retina (Jow, G.M. and Jeng, C.J. (2008) Neurosci. Lett. 431, 12.).
Scientific background
KV10.2 is a member of the Ether-a-go-go family of voltage-activated potassium channels. To date, the family includes eight members: the closely related KV10.1 protein, the KV12 (elk) (elk1, elk2, elk3) and the KV11 (erg) proteins (erg1, erg2, erg3).
The eag subfamily current is characterized by outward rectification without inactivation and slow activation kinetics. No specific pharmacological blocker has been described.
Tissue distribution of KV10.2 expression in rats is restricted to neuronal tissue with greater expression in the cortex and thalamus. In humans, KV10.2 mRNA was detected in heart and skeletal muscle in addition to brain. The physiological role of KV10.2 is currently unknown.
Lyophilized Powder
For research purposes only, not for human use
Last Update: 29/10/2024
Specifications
Citations
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