Name: Anti-KCNH6 (erg2) Antibody
Brand: Alomone Labs
SKU: APC-114

Overview

Cat #: APC-114

Alternative Name K_V11.2, Potassium voltage-gated channel subfamily H member 6, Ether-a-go-go-related channel 2, Eag-related protein 2, ERG-2, hERG2

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide TLNFVEFNLEKHRS(C), corresponding to amino acid residues 185-198 of human K_V11.2 (erg2) (Accession Q9H252). Intracellular, N-terminal part.

Accession (Uniprot) Number Q9H252

Gene ID 81033

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat, chicken - identical; mouse - 13/14 amino acid residues identical.

RRID AB_2039842.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.75 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ifc*, ip*

Western blot

Western blot analysis of rat brain (1 and 3) and cortex (2 and 4) lysate:
1,2. Anti-KCNH6 (erg2) Antibody (#APC-114), (1:200).
3,4. Anti-KCNH6 (erg2) Antibody, preincubated with KCNH6/erg2 Blocking Peptide (#BLP-PC114).

Immunohistochemistry

Expression of K_V11.2 (erg2) in rat hippocampus
Immunohistochemical staining of K_V11.2 in rat hippocampus using Anti-KCNH6 (erg2) Antibody (#APC-114). A. K_V11.2 appears as diffuse staining (green) that defines the boundary of the reticular nucleus of thalamus (arrow). B. Staining with mouse anti-parvalbumin (PV, red) demonstrates both neuronal and diffuse staining. C. Confocal merge of K_V11.2 and PV demonstrates overlap of the two markers labeling the reticular nucleus of the thalamus (orange).
Mouse vomeronasal organ (VNO) (1:250) (Hagendorf, S. et al. (2009) J. Neurosci. 29, 206.).

References

Shi. W.M. et al. (1997) J. Neurosci. 17, 9423.
Wimmers, S. et al. (2001) Pflugers Arch. 441, 450.
Schwarz, J.R. and Bauer, C.K. (2004) J. Cell. Mol. Med. 8, 22.

Scientific background

K_V11.2 (erg2) is a member of the ether-a-go-go (EAG) subfamily of voltage-dependent K⁺ channels. The erg subfamily includes the closely related proteins K_V11.1 (erg1) and K_V11.3 (erg3) that possess the signature structure of the voltage-dependent K⁺ channels: six membrane-spanning domains with intracellular N- and C-termini. As with all voltage-dependent K⁺ channels, the functional channel is a tetramer composed of four subunits. It has been suggested that the K_V11 subfamily members can form functional heteromultimers within the subfamily.

K_V11.2 produces currents characterized by strong inward rectification with slow activation and very rapid inactivation kinetics, which closely resemble those produced by the much studied channel K_V11.1.

The expression of K_V11.2 seems to be limited to the brain and the pituitary gland. The same is true for the K_V11.3 protein, while K_V11.1 is more widely expressed.

From a pharmacological point of view, K_V11.2 channels can be blocked by well characterized organic blockers such as the anti-arrhythmic drug E-4031. In addition, the peptide toxins Ergtoxin-1 and BeKm-1 block K_V11.2 with different potencies.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Alomone Labs is pleased to offer Anti-KCNH6 (erg2) Antibody (#APC-114) directed against a highly conserved epitope of the human K_V11.2 channel. The antibody can be used to detect K_V11.2 in rat samples using both western blotting and immunohistochemistry applications.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

More product citations

Hagendorf, S. et al. (2009) J. Neurosci. 29, 206.
Hardman, R.M. and Forsythe, I.D. (2009) J. Physiol. 587.11, 2487.

Specifications

Scientific Background

Specific Control Product

KCNH6/erg2 Blocking Peptide (#BLP-PC114) is the original antigen used for immunization during Anti-KCNH6 (erg2) Antibody (#APC-114) generation. The blocking peptide binds and ‘blocks’ Anti-KCNH6/erg2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

KCNH6/erg2 Blocking Peptide (#BLP-PC114)

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Anti-KCNH6 (erg2) Antibody