Overview
- Peptide CRQDIYQAVGEVKKD, corresponding to amino acid residues 859-873 of rat KV2.2 (Accession Q63099). Intracellular, C-terminus.
Western blot analysis of rat brain lysate:1. Anti-KV2.2 (KCNB2) Antibody (#APC-120), (1:2000).
2. Anti-KV2.2 (KCNB2) Antibody, preincubated with Kv2.2/KCNB2 Blocking Peptide (#BLP-PC120).
- Rat brain membranes (Kihira, Y. et al. (2010) J. Biol. Chem. 285, 15048.).
- Rat brain sections (Kihira, Y. et al. (2010) J. Biol. Chem. 285, 15048.).
- HEK-293 cells transfected with rabbit KV2.2 (Kyle, B. et al. (2011) Am. J. Physiol. 301, C1186.).
- Hwang, P.M. et al. (1992) Neuron 8, 473.
- Schmalz, F. et al. (1998) Am. J. Physiol. 274, G901.
- Blaine, J.T. and Ribera, A.B. (2001) J. Neurosci. 21, 1473.
- Escoubas, P. et al. (2002) Mol. Pharmacol. 62, 48.
KV2.2 is a member of the voltage-gated K+ channel superfamily. Together with the closely related KV2.1 protein they form the KV2 subfamily also known as Shab.
As with all KV channels, KV2.2 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N and C termini. The functional KV channel is a tetramer that can either be a homotetramer or a heteromer of KV2.2 and KV2.1 subunits.
Both KV2.2 and KV2.1 channels are known as delayed rectifiers that is, channels that are activated by changes in membrane potential (depolarization) but inactivate very slowly. The current they form is known as IK or IDR. Accessory subunits such as KChaP and the electrically silent a subunits KV8 and KV9 can modulate biochemical and biophysical properties of KV2.2.
KV2.2 is expressed in the brain and in peripheral tissues such as gastrointestinal and vascular smooth muscle. Physiologically, KV2.2 has a role in maintaining membrane potential and in modulating electrical excitability in neurons. In smooth muscle cells, KV2.2 is probably involved in maintaining the contractile capacity of the cells.
The pharmacology of the KV2.2 channel is somewhat limited. The channel is blocked by the classical K+ channel blockers tetraethylammonium (TEA) and 4-aminopyridine (4-AP). More recently, the small peptide toxin Stromatoxin-1 has been found to be a potent and more specific inhibitor of the KV2 subfamily.
Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the rat KV2.2 channel. Anti-KV2.2 (KCNB2) Antibody (#APC-120) can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize KV2.2 from rat, human, and mouse samples.
Applications
Citations
Powered by Bioz- Rat 832/13 cells expressing human KV2.2 (1:1000).
Jensen, M.V. et al. (2013) J. Biol. Chem. 288, 23128.
- Rat 832/13 cells expressing human KV2.2 (2 µg / 5x106 cells).
Jensen, M.V. et al. (2013) J. Biol. Chem. 288, 23128. - Rat brain membranes.
Kihira, Y. et al. (2010) J. Biol. Chem. 285, 15048.
- HEK-293 cells transfected with rabbit KV2.2.
Kyle, B. et al. (2011) Am. J. Physiol. 301, C1186.
- Tong, H. et al. (2013) J. Neurosci. 33, 9113.
- Hermanstyne, T.O. et al. (2012) J. Comp. Neurol. 518, 4298.
- Sanchez Ponce, D. et al. (2012) PLoS ONE 7, e48557.
- Johnston, J. et al. (2008) J. Physiol. 586.14, 3493.
