Name: Anti-KCNC3 (KV3.3) Antibody
Brand: Alomone Labs
SKU: APC-102

Overview

Cat #: APC-102

Alternative Name Potassium voltage-gated channel subfamily C member 3, KSHIIID, SCA13

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide KSPITPGSRGRYSRDRAC, corresponding to amino acid residues 701-718 of rat KCNC3 (Accession Q01956). Intracellular, C-terminus.

Accession (Uniprot) Number Q01956

Gene ID Q01956

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Human, mouse-identical.

RRID AB_2040170.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Specificity Recognizes only splice variant K_V3.3-1.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ic, if, ih, wb

May also work in: ifc*, ip*

Western blot

Western blotting of rat brain membranes:
1. Anti-KCNC3 (K_V3.3) Antibody (#APC-102), (1:200).
2. Anti-KCNC3 (K_V3.3) Antibody, preincubated with KCNC3/Kv3.3 Blocking Peptide (#BLP-PC102).

Immunohistochemistry

Rat brain sections.

Immunocytochemistry

Mouse spiral ganglia (SGNs) (Chen, W.C. and Davis R.L. (2006) Hear. Res. 222, 89.).

References

Rudy, B. and McBain C.J. (2001) Trends Neurosci. 24, 517.
Matsukawa, H. et al. (2003) J Neurosci. 23, 7677.

Scientific background

K_V3.3 is a member of the Shaw-type family of voltage-gated K⁺ channels that includes four members (K_V3.1-3.4).

K_V3 subfamily members inactivate very rapidly and therefore are thought to play a role in the repolarization of action potentials and to facilitate repetitive high frequency firing.

K_V3.3 is mostly localized in the brain, although it has been observed in vascular smooth muscle cells and eye epithelium. In the brain its expression pattern largely overlaps that of the K_V3.1 channel suggesting that they may form functional heteromers. Indeed, mouse knockouts of both K_V3.1 and K_V3.3, but not either channel alone, display severe motor defects.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat K_V3.3 channel. Anti-KCNC3 (K_V3.3) Antibody (#APC-102) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize K_V3.3 from rat, mouse and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

KO validation citations

Western blot of mouse brainstem samples and immunohistochemistry of mouse medial nucleus of the trapezoid body (MNTB). Tested in K_v3.3 knockout mice.
Choudhury, N. et al. (2020) J. Physiol. 598.11, 2199.

Immunocytochemistry citations

Mouse spiral ganglia (SGNs).
Chen, W.C. and Davis R.L. (2006) Hear. Res. 222, 89.

More product citations

Alonso-Espinaco, V. et al. (2008) Neuroscience 155, 1059.
Hurlock, E.C. et al. (2008) J. Neurosci. 28, 4640.
Cartwright, T. A. et al. (2007) Biochim. Biophys. Acta 1770, 666.
Kaneda, M. et al. (2007) J. Neurophysiol. 97, 4225.
Pannaccione, A. et al. (2007) Mol. Pharmacol. 72, 665.
Xia, F. et al. (2007) Endocrinol. 148, 2157.
Brooke, R.E. et al. (2004) Eur. J. Neurosci. 20, 3313.
Shevchenko, T. et al. (2004) J. Neurophysiol. 92, 3043.

Specifications

Scientific Background

Specific Control Product

KCNC3/Kv3.3 Blocking Peptide (#BLP-PC102) is the original antigen used for immunization during Anti-KCNC3 (K_V3.3) Antibody (#APC-102) generation. The blocking peptide binds and ‘blocks’ Anti-KCNC3/Kv3.3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

KCNC3/Kv3.3 Blocking Peptide (#BLP-PC102)

Shipping and Ordering Information

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Anti-KCNC3 (K_V3.3) Antibody