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Overview
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat KV3.3 channel. Anti-KCNC3 (KV3.3) Antibody (#APC-102) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize KV3.3 from rat, mouse and human samples.
Alternative Name Potassium voltage-gated channel subfamily C member 3, KSHIIID, SCA13
Accession (Uniprot) Number Q01956
Gene ID Q01956
For research purposes only, not for human use
Applications
Citations (12)
Citations
Powered by BiozKO validation citations
- Western blot of mouse brainstem samples and immunohistochemistry of mouse medial nucleus of the trapezoid body (MNTB). Tested in Kv3.3 knockout mice.
Choudhury, N. et al. (2020) J. Physiol. 598.11, 2199.
Immunocytochemistry citations
- Mouse spiral ganglia (SGNs).
Chen, W.C. and Davis R.L. (2006) Hear. Res. 222, 89.
More product citations
- Alonso-Espinaco, V. et al. (2008) Neuroscience 155, 1059.
- Hurlock, E.C. et al. (2008) J. Neurosci. 28, 4640.
- Cartwright, T. A. et al. (2007) Biochim. Biophys. Acta 1770, 666.
- Kaneda, M. et al. (2007) J. Neurophysiol. 97, 4225.
- Pannaccione, A. et al. (2007) Mol. Pharmacol. 72, 665.
- Xia, F. et al. (2007) Endocrinol. 148, 2157.
- Brooke, R.E. et al. (2004) Eur. J. Neurosci. 20, 3313.
- Shevchenko, T. et al. (2004) J. Neurophysiol. 92, 3043.
Specifications
Properties
Immunogen
- Peptide KSPITPGSRGRYSRDRAC, corresponding to amino acid residues 701-718 of rat KCNC3 (Accession Q01956). Intracellular, C-terminus.
Homology Human, mouse-identical.
RRID AB_2040170.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Recognizes only splice variant KV3.3-1.
Standard quality control of each lot Western blot analysis.
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Scientific Background
Scientific Background
Scientific background
KV3.3 is a member of the Shaw-type family of voltage-gated K+ channels that includes four members (KV3.1-3.4).
KV3 subfamily members inactivate very rapidly and therefore are thought to play a role in the repolarization of action potentials and to facilitate repetitive high frequency firing.
KV3.3 is mostly localized in the brain, although it has been observed in vascular smooth muscle cells and eye epithelium. In the brain its expression pattern largely overlaps that of the KV3.1 channel suggesting that they may form functional heteromers. Indeed, mouse knockouts of both KV3.1 and KV3.3, but not either channel alone, display severe motor defects.
References
- Rudy, B. and McBain C.J. (2001) Trends Neurosci. 24, 517.
- Matsukawa, H. et al. (2003) J Neurosci. 23, 7677.
Last Update: 25/03/2025
