Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ip*
Western blot
Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysates (lanes 2 and 4):
Voltage-gated K+ (Kv) channels are potassium selective multimeric channels that change their conformation in response to changes in the membrane potential. Kv channels play a central role in the determination of cellular excitability due to their contribution to the shape, duration and frequency of action potentials. A wide range of physiological activities are regulated via KV actions including cell signaling, proliferation and migration1.
Kv channels are divided into several subfamilies based in sequence homology. Members of the Kv5, Kv6, Kv8, and Kv9 subfamilies, are unable to form functional channels, even though they have the typical topology of a Kv α-subunit and have therefore been designated silent Kv (KvS) subunits.
KV6.4 (also known as KCNG4) is part of the electrically silent KV6 subfamily that includes four members Kv6.1-Kv6.42. Members of the Kv6 subfamily selectively interact with members of the Kv2 subfamily, forming functional Kv2/KvS heterotetramers that possess unique biophysical and pharmacological properties 2.
Heterotetramerization of KV6.4 with Kv2 results in the formation of functional channels. The stoichiometry of Kv2/Kv6 complex was recently found to be 2:2 stoichiometry with activation V1/2 of 5.7 ± 3.1 mV and inactivation V1/2 of -62.8 ± 1.6 mV3.
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot