Name: Anti-KCNQ1 (extracellular) Antibody
Brand: Alomone Labs
SKU: APC-168

Overview

Cat #: APC-168

Alternative Name K_V7.1, Voltage-gated potassium channel QKT subfamily member 1, KvLQT1, KCNA9

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide(C)EKDAVNESGRIEFG, corresponding to amino acid residues 284-297 of rat KCNQ1 (Accession Q9Z0N7). 3^rd extracellular loop.

Accession (Uniprot) Number Q9Z0N7

Gene ID 84020

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse – identical; human – 13/14 amino acid residues identical.

RRID AB_2756752.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ic, if, ifc, lci, wb

May also work in: ih*, ip*

Western blot

Western blot analysis of rat heart lysate (lanes 1 and 3) and mouse heart lysate (lanes 2 and 4):
1,2. Anti-KCNQ1 (extracellular) Antibody (#APC-168), (1:200).
3,4. Anti-KCNQ1 (extracellular) Antibody, preincubated with KCNQ1 (extracellular) Blocking Peptide (#BLP-PC168).

Indirect flow cytometry

Cell surface detection of KCNQ1 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:
^___ Cells.
^___ Cells + goat-anti-rabbit-FITC.
^___ Cells + Anti-KCNQ1 (extracellular) Antibody (#APC-168), (2.5μg) + goat-anti-rabbit-FITC.

Live cell imaging / Immunocytochemistry

Expression of KCNQ1 in Human COLO-205 cells
Cell surface detection of KCNQ1 in live intact human COLO-205 colon adenocarcinoma cells. A. Extracellular staining of cells with Anti-KCNQ1 (extracellular) Antibody (#APC-168), (1:50), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody (green). B. Live view of the cells. C. Merge of A and B.

References

Wang, Q. et al. (1996) Nat. Genet. 12, 17.
Grahammer, F. et al. (2001) Gastroenterology 120, 1363.
Mall, M. et al. (1998) Am. J. Physiol. 275, G1274.

Scientific background

KCNQ1 (K_V7.1) is part of a voltage-gated K⁺ family that includes 5 other members (KCNQ1-5).

The channel has been intensively studied since it was found that together with MinK (Isk) it underlay the cardiac I_ks current that controls the duration of the action potential of the human heart. Indeed mutations on either K_V7.1 or MinK can be responsible for long QT syndrome, a cardiac disorder that causes arrhythmias and sudden death.

Although K_V7.1 expression and function has been mainly investigated in relation with cardiac function the channel is widely distributed in epithelial tissues. There it has been implicated in physiological functions such as: regulation of acid secretion in the stomach and Cl^- secretion into the colon.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat KCNQ1 (K_V7.1). Anti-KCNQ1 (extracellular) Antibody (#APC-168) can be used in western blot and live cell imaging applications. It has been designed to recognize KCNQ1 from rat, human and mouse samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

KCNQ1 (extracellular) Blocking Peptide (#BLP-PC168) is the original antigen used for immunization during Anti-KCNQ1 (extracellular) Antibody (#APC-168) generation. The blocking peptide binds and ‘blocks’ Anti-KCNQ1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

KCNQ1 (extracellular) Blocking Peptide (#BLP-PC168)

Shipping and Ordering Information

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Anti-KCNQ1 (extracellular) Antibody