Name: Anti-KV8.1 (KCNV1) Antibody
Brand: Alomone Labs
SKU: APC-139

Overview

Cat #: APC-139

Alternative Name Potassium Voltage-Gated Channel Subfamily V Member 1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)KKDTDDQESQHESE, corresponding to amino acid residues 171 - 184 of rat KCNV1 (Accession P97557). Intracellular, N-terminus.

Accession (Uniprot) Number P97557

Gene ID 60326

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse – identical; Human - 13 out of 14 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preadsorption Control 1 µg peptide per 1 µg antibody

Standard quality control of each lot Western blot analysis.

Applications: wb

May also work in: ic*, ih*, ip*

Western blot

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
1-2. Anti-K_V8.1 (KCNV1) Antibody (#APC-139), (1:200).
3-4. Anti-K_V8.1 (KCNV1) Antibody, preincubated with K_V8.1 (KCNV1) Blocking Peptide (BLP-PC139).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
1. Anti-K_V8.1 (KCNV1) Antibody (#APC-139), (1:500).
2. Anti-K_V8.1 (KCNV1) Antibody, preincubated with K_V8.1 (KCNV1) Blocking Peptide (BLP-PC139).

References

De Lera Ruiz, M., et al (2015) J. Med. Chem. 58, 7093-7118.
Bocksteins, E., (2016) J Gen Physiol. 147, 105–125.

Scientific background

Voltage-gated K⁺ channels (K_V) are large and diverse ion channel family with more than 40 members found in all kingdoms of life. The activity of K_Vresults in the generation and propagation of membrane potential. A wide range of physiological activities are regulated via K_Vactions among them, cell signaling, proliferation and migration. K_V divided into 12 sub-families, K_V1- K_V12 based on their amino acid sequence homology¹.

The electrically silent voltage-gated K⁺ (K_VS) is a subfamily of four K_V(K_V5, K_V6, K_V8 and K_V9) that do not assembles into a functional homotetrameric channel. K_VS is known to generate a functional heterotetrameric complex with K_V2 subunits at the plasma membrane^1,2.

K_V8.1 is a member of the K_VS family that was first cloned in 1996 and share 40% identity with other K_Vchannels. Based on sequence homology K_V8.1 appear to contain six transmembrane segments (S1-S6), and cytoplasmic N and C termini. Heterotetrameric Kv2.1/K_V8.1 was found to have activation V_1/2 of 10 to 19 mV and inactivation V_1/2 of -63 mV. In recent studies Kv8.1 is found to be involved in epilepsy ².

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-K_V8.1 (KCNV1) Antibody (#APC-139) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot. It has been designed to recognize K_V8.1 from rat, mouse and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

K_V8.1 (KCNV1) Blocking Peptide (#BLP-PC139) is the original antigen used for immunization during Anti-K_V8.1 (KCNV1) Antibody (#APC-139) generation. The blocking peptide binds and ‘blocks’ Anti-K_V8.1 (KCNV1) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

K_V8.1 (KCNV1) Blocking Peptide (#BLP-PC139)

Shipping and Ordering Information

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Anti-K_V8.1 (KCNV1) Antibody