Name: Anti-LAT1 (SLC7A5) Antibody
Brand: Alomone Labs
SKU: ANT-105

Overview

Cat #: ANT-105

Alternative Name CD98, Large neutral amino acids transporter small subunit 1, L-type amino acid transporter 1, 4F2 light chain, 4F2LC, Integral membrane protein E16, MPE16, Solute carrier family 7 member 5, TA1, Tumor-associated protein 1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

Immunogen

Peptide (C)KDIGQGDASNLHQK, corresponding to amino acid residues 222-235 of rat LAT1 (Accession Q63016). 3^rd intracellular loop.

Accession (Uniprot) Number Q63016

Gene ID 50719

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse – 12/14 amino acid residues identical.

RRID AB_2756724.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Specificity Not recommended for use with human samples.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ih, wb

May also work in: ic*, ifc*, ip*

Western blot

Western blot analysis of rat placenta lysate (lanes 1 and 4), mouse testes lysate (lanes 2 and 5) and rat skeletal muscle membrane (lanes 3 and 6):
1-3. Anti-LAT1 (SLC7A5) Antibody (#ANT-105), (1:200).
4-6. Anti-LAT1 (SLC7A5) Antibody, preincubated with LAT1/SLC7A5 Blocking Peptide (#BLP-NT105).

Immunohistochemistry

Expression of LAT1/SLC7A5 in rat dorsal root ganglion.
Immunohistochemical staining of perfusion-fixed frozen rat dorsal root ganglion (DRG) sections with Anti-LAT1 (SLC7A5) Antibody (#ANT-105), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. LAT1 immunoreactivity (green) appeared in neuron profiles (arrows). B. Pre-incubation of the antibody with LAT1 (SLC7A5) Blocking Peptide (#BLP-NT105), suppressed staining. Cell nuclei are stained with DAPI (blue).

References

Kobayashi, N. et al. (2018) Sci. Rep. 8, 2785.
Hayase, S. et al. (2017) Oncol. Lett. 14, 7410.
Wagner, C.A. et al. (2001) Am. J. Physiol. 281, 1077.

Scientific background

L-type amino acid transporter 1 (LAT1) is responsible for mediating the transport of amino acids into highly proliferative cells. LAT1 is Na⁺-independent amino acid transporter that transports branched-chain and aromatic neutral amino acids including isoleucine, histidine, leucine, methionine, phenylalanine, tryptophan, tyrosine, methionine and valine. LAT1 forms heterodimeric complexes with the 4F2 cell-surface antigen heavy chain (4F2hc) via a disulfide bond ^1,2. Structurally, LAT1 has 12 membrane spanning domains with intracellular N- and C-terminal tails³.

LAT1 mRNA expression can be found in various tissues such as the brain, spleen, placenta, and testis. High protein levels are particularly detected in pancreatic islet. In cells, LAT1 is localized to the basolateral membrane of polarized epithelia^1,2.

Studies show that upregulation in LAT1 protein is linked and associated with cancer cell proliferation and is highly expressed in tumor cell lines including oral squamous cell carcinoma, esophageal carcinoma, gastric cancer, prostate cancer, non-small cell lung carcinoma, biliary tract cancer, pancreatic cancer, and breast cancer².

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-LAT1 (SLC7A5) Antibody (#ANT-105) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize SLC7A5 from rat and mouse samples. The antibody is unlikely to recognize the protein from human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

LAT1/SLC7A5 Blocking Peptide (#BLP-NT105) is the original antigen used for immunization during Anti-LAT1 (SLC7A5) Antibody (#ANT-105) generation. The blocking peptide binds and ‘blocks’ Anti-LAT1/SLC7A5 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

LAT1/SLC7A5 Blocking Peptide (#BLP-NT105)

Shipping and Ordering Information

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Anti-LAT1 (SLC7A5) Antibody