Overview
- Peptide (C)EDDGMKWLRIPSNVK, corresponding to amino acid residues 777 - 791 of mouse LEPR (Accession P48356). Extracellular, N-terminus.
Leptin Receptor (extracellular) Blocking Peptide (#BLP-LR039)
Western blot analysis of rat brain membranes (lanes 1 and 4), rat dorsal root ganglion lysate (lanes 2 and 5) and mouse brain membranes (lanes 3 and 6):1-3. Anti-Leptin Receptor (extracellular) Antibody (#ALR-039), (1:200).
4-6. Anti-Leptin Receptor (extracellular) Antibody, preincubated with Leptin Receptor (extracellular) Blocking Peptide (BLP-LR039).
Western blot analysis of rat liver lysates:1. Anti-Leptin Receptor (extracellular) Antibody (#ALR-039), (1:200).
2. Anti-Leptin Receptor (extracellular) Antibody, preincubated with Leptin Receptor (extracellular) Blocking Peptide (BLP-LR039).
Western blot analysis of mouse J774 macrophage cell line lysate (lanes 1 and 3) and mouse TK-1 T-cell lymphoma cell line lysate (lanes 2 and 4):1-2. Anti-Leptin Receptor (extracellular) Antibody (#ALR-039), (1:200).
3-4. Anti-Leptin Receptor (extracellular) Antibody, preincubated with Leptin Receptor (extracellular) Blocking Peptide (BLP-LR039).
Expression of Leptin Receptor in mouse olfactory bulb.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Leptin Receptor (extracellular) Antibody (#ALR-039), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Leptin Receptor immunoreactivity (green) appears in the mitral layer (M). B. Pre-incubation of the antibody with Leptin Receptor (extracellular) Blocking Peptide (BLP-LR039), suppressed staining. Cell nuclei are stained with DAPI (blue). M = mitral layer, G = glomerulus.
Expression of Leptin Receptor in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Leptin Receptor (extracellular) Antibody (#ALR-039), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Leptin Receptor immunoreactivity (green) appears in the pyramidal layer (P). B. Pre-incubation of the antibody with Leptin Receptor (extracellular) Blocking Peptide (BLP-LR039), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of Leptin Receptor in rat dorsal root ganglion (DRG).Immunohistochemical staining of perfusion-fixed frozen rat DRG sections with Anti-Leptin Receptor (extracellular) Antibody (#ALR-039), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Leptin Receptor immunoreactivity (green) appears in cell outlines (arrows). B. Pre-incubation of the antibody with Leptin Receptor (extracellular) Blocking Peptide (BLP-LR039), suppressed staining. Cell nuclei are stained with DAPI (blue).
Cell surface detection of Leptin Receptor by indirect flow cytometry in live intact mouse J774 macrophage cell line:___ Cells.
___ Cells + goat-anti-rabbit-PE.
___ Cells + Anti-Leptin Receptor (extracellular) Antibody (#ALR-039), (5μg) + goat-anti-rabbit-PE.
- Carpenter, B. et al. (2012) Structure. 20,3.
- Allison, M.B. and Myers, M.G. (2014) J. Endocrinol. 223,1.
Leptin receptor (LEP-R), also known as OB receptor (OB-R), HuB219, and CD295 is a receptor for the hormone leptin, which is involved in energy homeostasis and hunger/satiation cues.1
LEP-R, encoded by the LEPR gene, is composed of 1165 amino acids with the extracellular domain consisting an N-terminal cytokine receptor homology domain (CRH-1), an immunoglobulin-like (Ig) domain, a second CRH domain (CRH-2) which is also known as the leptin-binding domain (LBD) and two Fibronectin type III (FNIII) domains. LEP-R forms a high-affinity 1:1 ratio complex with leptin in solution, but does not form the 2:1 ratio complex associated with the small cytokine receptors, such as growth hormone receptor (GHR). The 1:1 ratio interaction occurs at leptin’s binding site II and can be blocked by mutations in this region. Mutations within the Ig domain of LEP-R and binding site III of leptin have been shown to inhibit transduction without disrupting receptor binding, indicating that the leptin-signaling complex forms a crossover arrangement between two leptin-LEP-R complexes.1
Leptin is produced in adipose tissue in proportion to triglyceride stores and serves as an indicator of long-term energy status. Leptin acts mainly in the brain, specifically the hypothalamus, where its action is integrated with that of other adipokines, gastrokines, and other signals to coordinate energy homeostasis. Once secreted into the circulation, leptin travels to the brain where it enters the CNS likely via the choroid plexus and circumventricular organs. In the brain, leptin acts by binding and activating LEP-R, which is primarily expressed on specialized subsets of neurons in certain hypothalamic and brainstem nuclei.2
Lack of leptin, such as in cases of starvation or genetic leptin deficiency, increases hunger while promoting an energy-sparing cascade of neuroendocrine and autonomic changes, including decreased sympathetic nervous system tone, thyroid function, growth, reproduction, and a variety of behavioral and physiological changes to respond appropriately to low stores of energy.2
Anti-Leptin Receptor (extracellular) Antibody (#ALR-039) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize Leptin Receptor from mouse and rat samples.
The antibody will not work with human samples.
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