Overview
- Peptide (C)DTGPTGIKYDLDRH, corresponding to amino acid residues 91-104 of human LRRC8A (Accession Q8IWT6). 1st extracellular loop.
- Mouse brain and rat lung lysates (1:200-1:1000).
- Western blot analysis of mouse brain lysate:1. Anti-LRRC8A (extracellular) Antibody (#AAC-001), (1:200).
2. Anti-LRRC8A (extracellular) Antibody, preincubated with LRRC8A (extracellular) Blocking Peptide (#BLP-AC001). - Western blot analysis of rat lung membranes:1. Anti-LRRC8A (extracellular) Antibody (#AAC-001), (1:200).
2. Anti-LRRC8A (extracellular) Antibody, preincubated with LRRC8A (extracellular) Blocking Peptide (#BLP-AC001).
- Mouse cerebellum and olfactory bulb (1:200).
Leucine-rich repeat-containing 8 A (LRRC8A, SWELL1) is an essential component of the volume-regulated anion channel (VRAC) that is activated by cell swelling and ionic strength. In response to swelling, mammalian cells release chloride and organic osmolytes through VRAC channels. LRRC8A serves as a sensor of ionic strength gradient in the absence of osmotic gradient. In addition, LRRC8A plays a role in the uptake of chemotherapeutic agents1-3.
LRRC8A protein structure contains 4 transmembrane segments and about 17 leucine-rich repeats (LRRs) at the cytoplasmic C-terminus3. VRAC channels are composed of the LRRC8A subunit and at least one of LRRC8B-E subunits. Deletion of LRRC8A greatly decreases the hypo-osmolarity-induced current1,3.
LRRC8A mRNA and protein are expressed in several neuronal tissues including brain stem ganglia, nodose ganglia and dorsal root ganglia. LRRC8A is also detected in the heart, aorta, and small intestine1.