Name: Anti-Lynx1 (extracellular) Antibody
Brand: Alomone Labs
SKU: ANC-021

Overview

Cat #: ANC-021

Alternative Name Ly-6/Neurotoxin-like protein 1, Endogenous prototoxin LYNX1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide CAYNGDN(S)FKPMR, corresponding to amino acid residues 26-38 of mouse Lynx1 (Accession P0DP60). Extracellular, N-terminus.

Accession (Uniprot) Number P0DP60

Gene ID 23936

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, rat – 12/13 amino acid residues identical; human – 11/13 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ifc, ih, wb

May also work in: ic*, ip*, lci*

Western blot

Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
1, 2. Anti-Lynx1 (extracellular) Antibody (#ANC-021), (1:200).
3, 4. Anti-Lynx1 (extracellular) Antibody, preincubated with Lynx1 (extracellular) Blocking Peptide (#BLP-NC021).
Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and human Jurkat T-cell leukemia cell line lysate (lanes 2 and 4):
1, 2. Anti-Lynx1 (extracellular) Antibody (#ANC-021), (1:200).
3, 4. Anti-Lynx1 (extracellular) Antibody, preincubated with Lynx1 (extracellular) Blocking Peptide (#BLP-NC021).

Immunohistochemistry

Expression of Lynx1 in rat dorsal raphe nucleus and substantia nigra pars compacta
Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Lynx1 (extracellular) Antibody (#ANC-021), (1:1000), followed by goat anti-rabbit-AlexaFluor-488. A. Lynx1 staining in the dorsal raphe nucleus (DRN), (green) is detected in cells of the medial DRN (MDRN, horizontal arrows) and in cells of the lateral DRN (LDRN), (vertical arrows). B. Lynx1 immunoreactivity in the substantia nigra pars compacta (SNC), (green) is observed in cells of the SNC (arrows). Cell nuclei are stained with DAPI (blue).

Indirect flow cytometry

Cell surface detection of Lynx1 in live intact human THP-1 monocytic leukemia cells:
^___ Cells.
^___ Cells + goat-anti-rabbit-FITC.
^___ Cells + Anti-Lynx1 (extracellular) Antibody (#ANC-021), (2.5 µg) + goat-anti-rabbit-FITC.

References

Miwa, J.M. et al. (1999) Neuron 23, 105.
Sadahiro, M. et al. (2016) J. Physiol. Paris 110, 29.
Morishita, H. et al. (2010) Science 330, 1238.
Vasilyeva, N.A. et al. (2017) Biochemistry (Mosc). 82, 1702.

Scientific background

Lynx1 is a member of the Ly-6/neurotoxin gene superfamily (Lynx family) of proteins that feature a three-looped toxin fold and a cysteine rich motif; structural characteristics that are shared with the snake venom toxin α-bungarotoxin¹.

Lynx1 is expressed in large projection neurons in the hippocampus, cortex, and cerebellum. Initial analysis of the functional consequence of genetic ablation of Lynx1 in mice revealed that Lynx1 is an allosteric modulator of various nicotinic receptors that affects their kinetics, decreases their agonist affinities, and increases their desensitization to overall limit their activation. Lynx1 can serve as a protective measure against neurotoxicity through agonist-induced overstimulation². In fact, deletion of Lynx1 eventually leads to neurodegeneration in aged mice³.

Collectively the findings regarding Lynx1 suggest a direct relationship between plasticity regulation and nicotinic signaling, and underline the significance of fine tuning of nicotinic tone to vastly control the development of brain functions.
Lynx1 activity was shown to be related to the pathophysiology of Alzheimer’s disease as well as cholinergic activity in healthy and tumor cells of bronchial epithelium⁴.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-Lynx1 (extracellular) Antibody (#ANC-021) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, indirect live cell flow cytometry, and immunohistochemistry applications. It has been designed to recognize Lynx1 from rat, mouse, and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

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Anti-Lynx1 (extracellular) Antibody

Overview

Application key:

Species reactivity key:

Applications

Citations

Specifications

Scientific Background

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