Name: Anti-MMGT1 Antibody
Brand: Alomone Labs
SKU: ANT-149

Overview

Cat #: ANT-149

Alternative Name Membrane magnesium transporter 1, ER membrane protein complex subunit 5, Transmembrane protein 32, Emc5, Tmem32

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

May also work in: h*

Immunogen

Peptide (C)NTSLKLRKFDSLRR, corresponding to amino acid residues 118-131 of rat MMGT1 (Accession B5DF51). Intracellular, C-terminus.

Accession (Uniprot) Number B5DF51

Gene ID 302864

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - identical; human - 12/14 amino acid residues identical.

RRID AB_2756734.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.85 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: wb

May also work in: ic*, ifc*, ih*, ip*

Western blot

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) heart lysates:
1,2. Anti-MMGT1 Antibody (#ANT-149), (1:400).
3,4. Anti-MMGT1 Antibody, preincubated with MMGT1 Blocking Peptide (#BLP-NT149).

References

Goytain, A. et al. (2008) Am. J. Physiol. Cell. Physiol. 294, C495.
Quamme, G.A. (2010) Am. J. Physiol. Cell. Physiol. 298, C407.

Scientific background

The MMgT gene family is comprised of two members: MMgT1 (membrane Mg²⁺ transporter 1) and MMgT2 (membrane Mg²⁺ transporter 2). Both proteins mediate Mg²⁺ uptake when expressed in Xenopus laevis oocytes. An increase in MMgT1 and MMgT2 transcripts is detected when there are low levels of Mg²⁺. There are two predicted transmembrane domains suggesting that the protein can form functional oligomers. It has been suggested that it may form homo- and, perhaps, heterooligomeric units.

MMgT1 can be found in the cell in golgi complex and post-golgi vesicles. The protein is expressed in a wide variety of tissues including in the liver and brain and more abundant expression in the heart muscle and kidney is observed. Very small amounts of transcripts are detected in the intestine and colon.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat membrane magnesium transporter 1. Anti-MMGT1 Antibody(#ANT-149) can be used in western blot analysis. It has been designed to recognize MMgT1 from mouse, rat and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

MMGT1 Blocking Peptide (#BLP-NT149) is the original antigen used for immunization during Anti-MMGT1 Antibody (#ANT-149) generation. The blocking peptide binds and ‘blocks’ Anti-MMGT1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

MMGT1 Blocking Peptide (#BLP-NT149)

Shipping and Ordering Information

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Anti-MMGT1 Antibody