Overview
- Peptide (C)SAQRKKEMVLSEK, corresponding to amino acid residues 28-40 of human MAGT1 (Accession Q9H0U3). Extracellular, N-terminus.
Western blot analysis of mouse colon lysate (lanes 1 and 4), rat brain lysate (lanes 2 and 5) and mouse brain lysate (lanes 3 and 6):1-3. Anti-MAGT1 (extracellular) Antibody (#ANT-197), (1:200).
4-6. Anti-MAGT1 (extracellular) Antibody, preincubated with MAGT1 (extracellular) Blocking Peptide (BLP-NT197).
Western blot analysis of human HepG2 hepatocarcinoma cell line lysate (lanes 1 and 4), human Jurkat T-cell leukemia cell line lysate (lanes 2 and 5) and human
THP-1 monocytic leukemia cell line lysate (lanes 3 and 6):1-3. Anti-MAGT1 (extracellular) Antibody (#ANT-197), (1:200).
4-6. Anti-MAGT1 (extracellular) Antibody, preincubated with MAGT1 (extracellular) Blocking Peptide (BLP-NT197).
Expression of MAGT1 in rat cortex.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-MAGT1 (extracellular) Antibody (#ANT-197), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the parietal cortex region showed MAGT1 immunoreactivity (green) in neuronal profiles (arrows). B. Pre-incubation of the antibody with MAGT1 (extracellular) Blocking Peptide (BLP-NT197), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of MAGT1 in rat supra-optic nucleus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-MAGT1 (extracellular) Antibody (#ANT-197), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. MAGT1 immunoreactivity (green) appears in neuronal profiles (arrows). B. Pre-incubation of the antibody with MAGT1 (extracellular) Blocking Peptide (BLP-NT197), suppressed staining. Cell nuclei are stained with DAPI (blue). OT = optic tract.
Cell surface detection of MAGT1 by indirect flow cytometry in live intact human Jurkat T-cell leukemia cells:___ Cells.
___ Cells + goat-anti-rabbit-FITC.
___ Cells + Anti-MAGT1 (extracellular) Antibody (#ANT-197), (5μg) + goat-anti-rabbit-FITC.
- Trapani et al. (2015), Magnes Res, 28, 46.
- Blommaert et al. (2019), Proc Natl Acad Sci USA, 116, 9865.
- Matsuda-Lennikov et al. (2019), J Biol Chem, 294, 13638.
Magnesium Transporter 1 (MAGT1) is a 335 amino acid peptide that is a part of the MAGT group of magnesium transporters. Magnesium (Mg2+) plays an essential cellular role, as a cofactor for ATP, nucleic acids, and numerous enzymes. It has been reported that it serves as a second messenger in intracellular signaling, and hence magnesium levels maintenance has a vast effect on cellular steady state 1.
In addition to its Mg2+ transporter activity, MAGT1 was also found to be a subunit of the oligosaccharyltransferase (OST) complex, through which it is responsible for protein glycosylation 2,3.
MAGT1 was shown to be related to the development of XMEN (X-linked immunodeficiency with magnesium defect, Epstein Barr Virus infection, and neoplasia) syndrome, for which Mg2+ supplementation has been shown to be beneficial. It is also related to atypical ATRX syndrome, intellectual disability and skin abnormalities.
Understanding the molecular basis of the involvement of magnesium and its channels in human pathogenesis can lead to the development of new medications for many diseases.
Anti-MAGT1 (extracellular) Antibody (#ANT-197) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize MAGT1 from mouse, rat and human samples.
Powered by Bioz
