Overview
- Peptide (C)ESHGEIMMTDLEKK, corresponding to amino acid residues 496-509 of mouse SLC47A1 (Accession Q8K0H1). 5th intracellular loop.
Western blot analysis of rat kidney (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat testis (lanes 3 and 6):1-3. Anti-SLC47A1 Antibody (#ANT-131), (1:200).
4-6. Anti-SLC47A1 Antibody, preincubated with SLC47A1 Blocking Peptide (#BLP-NT131).
Expression of SLC47A1 (MATE1) in rat parietal cortex.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SLC47A1 Antibody (#ANT-131), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. SLC47A1 (MATE1) immunoreactivity (green) appears in pyramidal neurons in the parietal cortex (arrows). B. Pre-incubation of the antibody with SLC47A1 Blocking Peptide (#BLP-NT131), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Lee, J.H. et al. (2014) J. Agric. Food Chem. 62, 9690.
- Wright, S.H. et al. (1995) Pflugers Arch. Eur. J .Physiol. 429, 313.
- Jong N.N. and McKeage, M.J. (2014) Biopharm. Drug Dispos. 35, 450.
- Maron, L.G. et al. (2013) Proc. Natl. Acad. Sci. U.S.A. 110, 5241.
- Wittwer, M.B. et al. (2013) J. Med. Chem. 56, 781.
MATE1 (SLC47A1) and MATE2 (SLC47A2) are multidrug and toxin extrusion transporter 1 and 2 function as cationic pumps in the kidneys, liver, and intestine1. Both MATEs excrete molecules with a positive net-charge from renal tubular epithelial cells in the kidney (where they are co-expressed with organic anion- and cation-transporters; OATs and OCTs), and into the urine. In the liver, MATE1, but not MATE2, regulates the transportation of tetraethylammonium (TEA) and 1-methyl-4-phenylpyridinium (MPP) – again, by cation-powered pumps2.
Fairly recent X-ray crystallography has revealed that mammalian MATE1 has 13 transmembrane helices and a long cytoplasmic tail. Cysteine and histidine residues are responsible for the protein's functionality1.
Overexpression of MATE1 is helpful in the delivery of metal-based anti-cancer drugs, such as cisplatin and oxaliplatin, as it effectively accumulates platinum intracellularly3. Furthermore, a rare increase in copy number of MATE1 in maize protects the plant from aluminum (Al) toxicity4. On the other hand, inhibition of MATE1 may lead to potentially dangerous drug-drug interactions, due to damaged drug extrusion regulation5.
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of mouse Multidrug and toxin extrusion protein 1 (MATE1, SLC47A1). Anti-SLC47A1 Antibody (#ANT-131) can be used in western blot analysis. It has been designed to recognize SLC47A1 from rat and mouse samples. The antibody will not recognize SLC47A1 from human samples.
Powered by Bioz
