Overview
- Peptide (C)RNMGYLKPRGSFE, corresponding to amino acid residues 87-99 of human MC2R (Accession Q01718). 1st extracellular loop.
Western blot analysis of rat adrenal lysate:1. Anti-MC2 Receptor (extracellular) Antibody (#AMR-022), (1:400).
2. Anti-MC2 Receptor (extracellular) Antibody, preincubated with MC2 Receptor (extracellular) Blocking Peptide (#BLP-MR022).
Expression of MC2R in rat adrenal glandImmunohistochemical staining of paraffin embedded rat adrenal cortex section using Anti-MC2 Receptor (extracellular) Antibody (#AMR-022), (1:100). Shown are the capsule (C), the zona glomerulosa (ZG) and the zona fasciculata (ZF) of the adrenal cortex. Note that the staining (brown) is specific for cells of the adrenal gland while no staining is present in the dense connective tissue of the capsule. Color reaction was obtained with DAB. H&E is used as the counterstain.
- Abdel-Malek, Z.A. (2001) Cell. Mol. Life Sci. 58, 434.
- Gantz, I. and Fong, T.M. (2003) Am. J. Physiol. Endocrinol. Metab. 284, E468.
- Wikberg, J.E.S. et al. (2000) Pharm. Res. 42, 393.
- Elias, L.L. et al. (1999) J. Clin. Endocrinol. Metab. 84, 2766.
Melanocortin receptor 2 (MC2R) belongs to a five-member receptor family known as the melanocortin receptors. The melanocortin receptors are members of the 7 transmembrane domain, G-protein coupled receptor (GPCR) superfamily.
The receptors' ligands, the melanocortins, are a group of structurally derived peptides consisting of α-, β- and γ melanocyte stimulating hormone (α, β, γ-MSH) and the adrenocorticotropic hormone (ACTH), all of which are derived from the post-translational processing of a common precursor peptide, proopiomelanocortin (POMC).1,2,3
One of the most salient features of the melanocortin signaling system is the presence of two endogenous antagonists; proteins that bind specifically to the receptor but instead of activating it have an inhibitory effect. The antagonist proteins are termed agouti (or agouti signaling protein, ASP) and agouti-related protein (AGRP).4
All five melanocortin receptors bind their agonists (the melanocortins) and their endogenous antagonists (agouti and AGRP) with different affinities.
MC2R can also be described as the ACTH receptor as it is exclusively activated by the ACTH hormone. In fact, MC2R mediates all the physiological effects of ACTH in the adrenal cortex, that is, the regulation and production of steroids, their circadian variation and their stress-related fluctuations.1,2,3 In addition, mutations that result in MC2R functional impairment are associated with familial glucocorticoid deficiency.4
The receptor transduces signals via Gs resulting in the activation of adenylate cyclase and production of cAMP.
Alomone Labs is pleased to offer a highly specific antibody directed against the first extracellular loop of the human melanocortin receptor 2. Anti-MC2 Receptor (extracellular) Antibody (#AMR-022) can be used in western blot and immunohistochemistry applications. It has been designed to recognize MC2R from human, mouse, and rat samples.
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