Overview
- Peptide (C)RYNSVRQRTSMKG, corresponding to amino acid residues 224-236 of mouse MC5R (Accession P41149). 3rd intracellular loop.
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-MC5 Receptor Antibody (#AMR-025), (1:200).
3,4. Anti-MC5 Receptor Antibody, preincubated with MC5 Receptor Blocking Peptide (#BLP-MR025).
Expression of MC5R in rat skinImmunohistochemical staining of paraffin embedded rat skin section using Anti-MC5 Receptor Antibody (#AMR-025), (1:100). MC5R staining is present in sebaceous glands. Hematoxilin is used as the counterstain.
- Abdel-Malek, Z.A. (2001) Cell. Mol. Life Sci. 58, 434.
- Gantz, I. and Fong, T.M. (2003) Am. J. Physiol. 284, E468.
- Wikberg, J.E. et al. (2000) Pharmacol. Res. 42, 393.
- Chen, W. et al. (1997) Cell 91, 789.
Melanocortin receptor 5 (MC5R) is one of five members of the melanocortin receptor family, which belongs to the 7-transmembrane domain, G-Protein Coupled Receptor (GPCR) superfamily.
The ligands of these receptors, the melanocortins, are a group of structurally-related peptides comprising the α-, β-, and γ-melanocyte-stimulating hormone (α-, β-, γ-MSH) and the adrenocorticotropic hormone (ACTH), all of which are derived from post-translational processing of a common precursor peptide, proopiomelanocortin (POMC) 1,2,3.
One of the salient features of the melanocortin signaling system is the existence of two endogenous antagonists, proteins that bind specifically to the receptor and have an inhibitory effect. These antagonist proteins are termed agouti (or agouti signaling protein, ASP) and agouti-related protein (AGRP) 1.
All five melanocortin receptors bind their agonists (the melanocortins) and their endogenous antagonists (agouti/ASP and AGRP) with differing affinities. The order of potency for MC5R activation is α-MSH > ACTH = β-MSH >> γ-MSH while AGRP works as an endogenous antagonists of MC5R1,2,3.
MC5R is mostly expressed in peripheral tissues such as skeletal muscle, bone marrow, skin tissues particularly in sebaceous glands and exocrine glands such as salivary glands1.
The best understood function of Melanocortin Receptor 5 is in the regulation of exocrine glands since MC5R-deficient mice exhibit severely defective water repulsion and thermoregulation due to decreased production of sebaceous lipids4.
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the mouse melanocortin receptor 5. Anti-MC5 Receptor Antibody (#AMR-025) can be used in western blot and immunohistochemical applications. It has been designed to recognize MC5R from mouse, human and rat samples.
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