Overview
- Peptide (C)KPPIKRQDGELVGYR, corresponding to amino acid residues 402 - 416 of mouse MERTK (Accession Q60805). Extracellular, N-terminus.
Cell surface detection of MERTK by direct flow cytometry in live intact mouse J774 macrophage cells:___ Cells.
___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
___ Cells + Anti-MERTK (extracellular)-FITC Antibody (#ATR-033-F), (2.5µg).
Cell surface detection of MERTK by direct flow cytometry in live intact mouse BV-2 microglia cells:___ Cells.
___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
___ Cells + Anti-MERTK (extracellular)-FITC Antibody (#ATR-033-F), (2.5µg).
Cell surface detection of MERTK by direct flow cytometry in live intact human MEG-01 megakaryoblastic leukemia cells:___ Cells.
___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
___ Cells + Anti-MERTK (extracellular)-FITC Antibody (#ATR-033-F), (5µg).
- Graham, D.K. et al. (2014) Nat. Rev. Cancer 14, 769.
- Kasikara, C. et al. (2017) Mol. Cancer Res. 15, 753.
- Huelse, J.M. et al. (2020) Pharmacol. Ther. 213, 107577.
- Cummings, C.T. et al. (2013) Clin. Cancer Res. 19, 5275.
Myeloid-epithelial-reproductive tyrosine kinase, MERTK, is a receptor tyrosine kinase of the TAM (Tyro3, Axl and MERTK) family, expressed in numerous cancers1.
The extracellular N-terminal domain contains two immunoglobulin-like and two fibronectin 3 domains, followed by a hydrophobic single pass transmembrane domain, and a cytoplasmic C-terminal tail containing a tyrosine kinase domain1,2.
Auto-phosphorylation induced by ligand binding influences a range of downstream signaling pathways and cellular functions2. The physiological roles of MERTK include regulation of tissue homeostasis and repair, innate immune control, and platelet aggregation. However, aberrant expression of this protein promotes neoplasia, cell cycle progression, proliferation and tumor growth, resistance to apoptosis, and promotion of tumor metastases.
MERTK is involved in the activation of several canonic oncogenic signaling pathways in various types of cancers including: leukemia, non-small cell lung cancer, glioblastoma, melanoma, prostate cancer, breast cancer, colon cancer and gastric cancer1,3,4.
Due to its pivotal role in cancer development and metastasis, multiple therapeutic approaches are currently in development to inhibit MERTK, including ligand "traps" and small-molecule tyrosine kinase inhibitors4.
Anti-MERTK (extracellular) Antibody (#ATR-033) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize MERTK from rat, mouse and human samples.
Anti-MERTK (extracellular)-FITC Antibody (#ATR-033-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize MERTK from rat, mouse and human samples.
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