Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Reconstitution 0.2 ml double distilled water (DDW).
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
Rat skeletal muscle and brain lysates. Mouse kidney lysates (1:200-1:1000).
Western blot analysis of rat skeletal muscle (lanes 1 and 4), mouse kidney (lanes 2 and 5) and rat brain (lanes 3 and 6) lysates:
Mitochondrial Ca2+ uptake was found to be mediated by the mitochondrial Ca2+ uniporter (MCU). MCU is a ruthenium‐red‐sensitive and highly selective Ca2+ ion channel that uptakes massive amount of Ca2+ across the inner membrane1. MICU1 is a ~54 kDa. protein with an amino‐terminal mitochondrial targeting sequence, a predicted transmembrane helix, and a cytosolic C‐terminus containing two classical EF‐hand Ca2+‐binding domains2.
Mitochondrial calcium uptake 1 (MICU1) protein was identified as an essential element of mitochondrial Ca2+ uptake. MCU initially takes up Ca2+ very rapidly, but when [Ca2+] inevitably increases inside the matrix, MICU1 binds Ca2+ through its EF‐hand domains, exerting an inhibitory role on MCU‐dependent Ca2+ entry3.
RNA expression of MICU1 is strongly correlated across a variety of tissues including: stomach, kidney, small and large intestine, cerebral cortex, cerebellum, hypothalamus, spinal cord and skeletal muscles4.
Loss-of-function mutations in MICU1 causes a brain and muscle disorder linked to primary alterations in mitochondrial Ca2+ signaling5.
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Western blot analysis of rat skeletal muscle (lanes 1 and 4), mouse kidney (lanes 2 and 5) and rat brain (lanes 3 and 6) lysates:1-3. Anti-MICU1 Antibody (#ACC-322), (1:200).
