Name: Anti-MCU Antibody
Brand: Alomone Labs
SKU: ACC-328

Overview

Cat #: ACC-328

Alternative Name Mitochondrial calcium uniporter, HsMCU, Coiled-coil domain-containing protein 109A, Ccdc109a, C10orf42

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide CIEQHQLNKERELIER, corresponding to amino acid residues 191-206 of human mitochondrial calcium uniporter (Accession Q8NE86). N-terminus, mitochondrial matrix.

Accession (Uniprot) Number Q8NE86

Gene ID 90550

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat, mouse – 15/16 amino acid residues identical.

RRID AB_2756559.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ih, wb

May also work in: ic*, ifc*, ip*

Western blot

Western blot analysis of rat kidney (lanes 1 and 5), rat skeletal muscle (lanes 2 and 6), rat brain (lanes 3 and 7) and mouse brain (lanes 4 and 8) lysates:
1-4. Anti-MCU Antibody (#ACC-328), (1:200).
5-8. Anti-MCU Antibody, preincubated with MCU Blocking Peptide (#BLP-CC328).

Immunohistochemistry

Expression of MCU in mouse midbrain.
Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-MCU Antibody (#ACC-328), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the mouse substantia nigra region, showed immunoreactivity (green) in neuronal profiles in the substantia nigra pars compacta (SNC, horizontal arrows). B. Pre-incubation of the antibody with MCU Blocking Peptide (BLP-CC328), suppressed staining. Cell nuclei are stained with DAPI (blue). SNR = substantia nigra pars reticulata.

References

Mammucari, C. et al. (2017) Front. Oncol. 7, 139.
Kon, N. et al. (2017) Cell Death Discov. 3, 17045.
Liao, Y. et al. (2017) Int. J. Mol. Sci. 18, 2.

Scientific background

Mitochondrial calcium uniporter (MCU) is a 480 kDa mitochondrial inner membrane protein, a multimer that forms a calcium (Ca²⁺) channel in the lipid bilayer. MCU is one of the most important and highly selective channels responsible for mitochondrial calcium uptake. This uptake plays an important role in cell energy balance and in cell fate determination.

The MCU protein is comprised of Mcu which functions as the pore-forming and calcium-conducting subunit, encoded by the Ccdc109a gene. The other units- MICU1 (calcium uptake protein 1, mitochondrial), MICU2 (calcium uptake protein 2, mitochondrial), EMRE (essential MCU regulator, mitochondrial), MCUR1 (mitochondrial calcium uniporter regulator 1) and MCUb (calcium uniporter regulatory subunit MCUb, mitochondrial) all function as regulatory subunits^1-3.

Studies have demonstrated that knockdown of Mcu uniport in isolated cardiomyocytes enhances peak levels in cytosolic Ca²⁺ oscillation, resulting in an increase of muscle contraction².

Mcu pore-forming subunit is a homo-oligomer with two coil-coiled domains and two transmembrane domains (TM) separated by a short hydrophilic loop enriched in acidic amino acids. The second transmembrane helices from five Mcu subunits form a hydrophilic pore in the inner membrane of the mitochondria. The DXXE motif forms the pore entrance in the inter-membrane space. The N- and the C-terminus of MCU face the matrix^1,3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-MCU Antibody (#ACC-328) is a highly selective antibody directed against an epitope of the human mitochondrial calcium uniporter. The antibody can be used in western blot analysis. It has been designed to recognize MCU from human, mouse, and rat samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

MCU Blocking Peptide (#BLP-CC328) is the original antigen used for immunization during Anti-MCU Antibody (#ACC-328) generation. The blocking peptide binds and ‘blocks’ Anti-MCU primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

MCU Blocking Peptide (#BLP-CC328)

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Anti-MCU Antibody