Overview
- Peptide (C)GRRYQSTVEAYK, corresponding to amino acid residues 153-164 of rat mitochondrial uncoupling protein 2 (UCP2) (Accession P56500).
Western blot analysis of mouse heart (lanes 1 and 5), rat skeletal muscle (lanes 2 and 6), rat adipose tissue (lanes 3 and 7) and rat pancreas (lanes 4 and 8):1-4. Anti-UCP2 Antibody (#ANT-128), (1:200).
5-8. Anti-UCP2 Antibody, preincubated with UCP2 Blocking Peptide (#BLP-NT128).
Western blot analysis of mouse muscle myoblast (C2C12), human brain glioblastoma (U-87 MG), human colorectal adenocarcinoma (HT-29) cell lines:1-3. Anti-UCP2 Antibody (#ANT-128), (1:200).
4-6. Anti-UCP2 Antibody, preincubated with UCP2 Blocking Peptide (#BLP-NT128).
Expression of UCP2 in mouse hypothalamus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-UCP2 Antibody (#ANT-128), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. UCP2 immunoreactivity (green) appears in neuron outlines (arrows). B. Pre-incubation of the antibody with UCP2 Blocking Peptide (#BLP-NT128), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = 3rd ventricle.
Expression of UCP2 in rat hypothalamus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-UCP2 Antibody (#ANT-128), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. UCP2 immunoreactivity (green) appears in neuron outlines (arrows). B. Pre-incubation of the antibody with UCP2 Blocking Peptide (#BLP-NT128), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = 3rd ventricle.
Expression of Mitochondrial uncoupling protein 2 (UCP2) in mouse C2C12 cells.Immunocytochemical staining of mouse muscle myoblast (C2C12) fixed, permeabilized cells. Intracellular staining of cells with Anti-UCP2 Antibody (#ANT-128), (green), (1:50-1:200) followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. A. Magnitude 1x40 (1:50). B-C. Magnitude 1x100 (1:200). DAPI is used as the counterstain (blue).
- Palmieri, F. (2004) Pflugers Arch. 447, 689.
Mitochondrial carriers (MCs) transport a variety of metabolites across the inner mitochondrial membrane. All MCs belong to the same family since their polypeptide chains consist of three tandemly related sequences of about 100 amino acids. MCs are encoded in humans by SLC25 genes.
UCP2 (Uncoupling protein 2) is a member of the MC family encoded by the SLC25A8 gene. It is expressed on the inner mitochondrial membrane of cells in the lungs, kidneys, spleen, heart and mainly in the hypothalamus, pituitary and brain stem. The expression of UCP2 is upregulated by fatty acids, prostaglandins, TNFα and other cytokines. UCP2 is involved in regulation of body temperature and the control of oxygen radical production.
Each monomer of UCP2 has six helices crossing the cell membrane connected by hydrophilic loops with both the N- and C- termini on the cytosolic side of the inner mitochondrial membrane. UCP2 catalyzes the unidirectional transport of H+. Charge balance can be achieved by cotransport (symport) of solutes, counter-transport of solutes and uniport of electroneutral metabolites. The transport of H+ is stimulated by fatty acids and inhibited by purine nucleotides. In addition, activation of H+ transport by superoxidase has been observed.
Recent studies suggest that UCP2 and other uncoupling proteins are involved in pathologies such as obesity, type-2 diabetes and atherosclerosis. Mice lacking UCP2 have been shown to over-produce reactive oxygen but not to gain weight or be insensitive to cold stimuli1.
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat mitochondrial uncoupling protein 2 (UCP2). Anti-UCP2 Antibody (#ANT-128) can be used in western blot and immunocytochemistry applications. It has been designed to recognize UCP2 from human, rat and mouse samples.
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