Name: Anti-MRGPRD (GPCR TGR7) Antibody
Brand: Alomone Labs
SKU: AMR-061

Overview

Cat #: AMR-061

Alternative Name Mrgd, Mas-related G-protein coupled receptor member D, G-protein coupled receptor TGR7, β-Alanine receptor

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

Immunogen

Peptide (C)KSHRLQESLGAVLGR, corresponding to amino acid residues 285-299 of rat MRGPRD (Accession Q7TN41). Intracellular, C-terminus.

Accession (Uniprot) Number Q7TN41

Gene ID 293648

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - identical. It will not recognize MRGPRD from human samples

RRID AB_2756664.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Specificity Not recommended for human samples.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ifc*, ip*

Western blot

Western blot analysis of rat dorsal root ganglion (lanes 1 and 4), mouse testis (lanes 2 and 5) and rat testis (lanes 3 and 6) lysates:
1-3. Anti-MRGPRD (GPCR TGR7) Antibody (#AMR-061), (1:400).
4-6. Anti-MRGPRD (GPCR TGR7) Antibody, preincubated with MRGPRD/GPCR TGR7 Blocking Peptide (#BLP-MR061).

Immunohistochemistry

Expression of MRGD in rat DRG
Immunohistochemical staining of rat dorsal root ganglion (DRG) sections using Anti-MRGPRD (GPCR TGR7) Antibody (#AMR-061), (1:200), followed by goat-anti rabbit-AlexaFluor-488. MRGD staining (green) appears in several DRGs (arrows). Cell nuclei are stained with DAPI (blue).
Rat jaw sections (Byers, M.R. and Cornel, L.M. (2018) Arch. Oral Biol. 85, 84.).

References

Uno, M. et al. (2012) J. Biomed. Biotechnol. 2012, 816159.
Wang, H. et al. (2009) J. Neurosci. 29, 13202.
Zylka, M.J. et al. (2003) Proc. Natl. Acad. Sci. USA. 100, 10043.
Rau, K.K. et al. (2009) J. Neurosci. 29, 8612.
Crozier, R.A. et al. (2007) J. Neurosci. 27, 4492.

Scientific background

Mas-related G-protein coupled receptor member D (MRGPRD) is expressed in the dorsal root ganglia, specifically in non-peptidergic, small-diameter, IB4+ C-fiber neurons that innervate the outer layers of the mammalian skin. They transmit polymodal nociceptive information from the skin epidermis to the substantia gelatinosa of the spinal cord.

MRGPRD receptor expressing neurons are essential for the full expression of mechanical nociception. Thus, MRGPRD represents an attractive therapeutic target for the development of agents for treating pain^1,2.

The Mrg family in mice is comprised of six single-copy genes: MrgD, MrgE, MrgF/RTA, MrgG, MrgH/GPR90, and MAS1. In addition, there are three large subfamilies: MrgA, MrgB,and MrgC³.

MRGPRD receptor is activated by β-alanine and is able to inhibit KCNQ/M-type potassium channels and to increase excitability of sensory neurons^4,5. MRGPRD has the ability to form heterodimers and is encoded by the TGR7 gene. This receptor is co-expressed with two nociceptors, including purinergic receptor (P2X3) and a vanilloid receptor (VR1)⁵.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-MRGPRD (GPCR TGR7) Antibody (#AMR-061) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize MRGPRD from rat and mouse samples. The antibody will not recognize MRGPRD from human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

KO validation citations

Immunohistochemistry of mouse colon. Tested in MRGPRD knockout mice.
Bautzova, T. et al. (2018) Sci. Signal. 11, eaal2171.

Immunohistochemistry citations

Rat jaw sections.
Byers, M.R. and Cornel, L.M. (2018) Arch. Oral Biol. 85, 84.

Specifications

Scientific Background

Specific Control Product

MRGPRD/GPCR TGR7 Blocking Peptide (#BLP-MR061) is the original antigen used for immunization during Anti-MRGPRD (GPCR TGR7) Antibody (#AMR-061) generation. The blocking peptide binds and ‘blocks’ Anti-MRGPRD/GPCR TGR7 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

MRGPRD/GPCR TGR7 Blocking Peptide (#BLP-MR061)