Name: Anti-MRGPRE Antibody
Brand: Alomone Labs
SKU: AMR-062

Overview

Cat #: AMR-062

Alternative Name Mas-related G-protein coupled receptor member E, GPCR MRGE, GPR167

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)RVERGPERHQPRG, corresponding to amino acid residues 197 - 209 of rat MRGPRE (Accession Q7TN40). 3^rd intracellular loop.

Accession (Uniprot) Number Q7TN40

Gene ID 404660

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse – identical; human – 10/13 amino acid residues identical.

RRID AB_2756665.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ifc*, ip*

Western blot

Western blot analysis of rat dorsal root ganglion lysate (lanes 1 and 4), mouse (lanes 2 and 5) and rat (lanes 3 and 6) brain membranes:
1-3. Anti-MRGPRE Antibody (#AMR-062), (1:400).
4-6. Anti-MRGPRE Antibody, preincubated with MRGPRE Blocking Peptide (#BLP-MR062).
Western blot analysis of human U-87 MG glioblastoma:
1. Anti-MRGPRE Antibody (#AMR-062), (1:400).
2. Anti-MRGPRE Antibody, preincubated with MRGPRE Blocking Peptide (#BLP-MR062).

Immunohistochemistry

Expression of MRGE in rat hippocampus
Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-MRGPRE Antibody (#AMR-062), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. MRGE staining (green) in hippocampal dentate gyrus is detected in astrocytes (arrows). Cell nuclei are stained with DAPI (blue).

References

Bader, M. et al. (2014) Pharmacol. Rev. 6, 1080.
Solinski, H.J. et al. (2014) Pharmacol. Rev. 66, 570.

Scientific background

The Mas-related G protein-coupled receptors (MRGPRs), formerly called Mas-related genes (MRGs) includes nine members: MrgprA, B, C, D, E, F, G, H, and the primate-specific MRGPRX^1,2.

MRGPRE consist of only one receptor per species and is conserved in rodents and primates². MRGPRE structure contains a Cys-Cys bridge from transmembrane domain (TM) 3, an “ionic-lock” known to inhibit activation and activity in some class A GPCRs^1,2.

Human and mouse MRGPRE is highly expressed in neurons of the brain and mast cells. Transcripts are also found in medium and large diameter neurons of human dorsal root ganglia (DRG) sections and in other areas of the central nervous system, including cerebral cortex, hippocampus, spinal cord, and cerebellum. MRGPRE was also found in human, mouse, and rat placenta tissue and in enteric neurons^1,2_.

Mouse lacking the MRGPRE protein showed decreased nociceptive behavior and significant deficits in the induction of mechanical allodynia after chronic constriction injury².

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-MRGPRE Antibody (#AMR-062) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize MRGPRE from rat, human, and mouse samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

MRGPRE Blocking Peptide (#BLP-MR062) is the original antigen used for immunization during Anti-MRGPRE Antibody (#AMR-062) generation. The blocking peptide binds and ‘blocks’ Anti-MRGPRE primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

MRGPRE Blocking Peptide (#BLP-MR062)