Name: Anti-SCN2A (NaV1.2)-ATTO Fluor-594 Antibody
Brand: Alomone Labs
SKU: ASC-002-AR

Overview

Cat #: ASC-002-AR

Alternative Name BII, Brain type II Na⁺ channel, Sodium channel protein type 2 subunit alpha

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)ASAESRDFSGAGGIGVFSE, corresponding to amino acid residues 467-485 of rat Na_V1.2 (Accession P04775). Intracellular loop between domains I and II.

Accession (Uniprot) Number P04775

Gene ID 24766

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Human, mouse – identical.

RRID AB_2341065.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Label ATTO-594. Maximum absorption 601 nm; maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label is related to the Rhodamine dyes and can be used with filters used to detect Texas Red and Alexa-594.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #ASC-002), and immunohistochemistry (labeled antibody).

Applications: if, ih

May also work in: ic*

Immunohistochemistry

Expression of Na_V1.2 in rat hippocampus
Immunohistochemical staining of rat hippocampus using Anti-SCN2A (Na_V1.2)-ATTO Fluor-594 Antibody (#ASC-002-AR). A. Staining of Na_V1.2 (red) appears in the stratum radiatum (CA3-SR) apical dendrites (arrow) of pyramidal neurons (Pyr). B. DAPI is used as the counterstain (blue). C. Merge images of A and B.

References

Wu, L. et al. (2002) NeuroReport 13, 2547.
Fang, X. et al. (2002) J. Neurosci. 22, 7425.
Fjell, J. et al. (2000) NeuroReport 11, 199.
Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27.
Lai, J. et al. (2003) Curr. Opin. Neurobiol. 13, 291.
Isom, L.L. (2001) Neuroscientist 7, 42.
Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 575.
Catterall, W.A. et al. (2008) J. Neurosci. 28, 11768.

Scientific background

Voltage-gated sodium channels (Na_V) are essential for the generation of action potentials and for cell excitability.¹ Na_V channels are activated in response to depolarization and selectively allow flow of Na⁺ ions. To date, nine Na_V α subunits have been cloned and named Na_V1.1-Na_V1.9.^4-5 The Na_V channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive (Na_V1.1, Na_V1.2, Na_V1.3, Na_V1.4, Na_V1.6 and Na_V1.7) and TTX-resistant (Na_V1.5, Na_V1.8 and Na_V1.9).^2-3

Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain β₁ through β₄ subunits, whereas sodium channels in adult skeletal muscle have only the β₁ subunit.^6,7

Na_V1.2, also known as SCN2A, is primarily expressed in the central nervous system (CNS) and is localized in unmyelinated or premyelinated axons and dendrites. Mutations in the Na_V1.2 channel have been identified in different types of epilepsy.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-SCN2A (Na_V1.2) Antibody (#ASC-002) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize Na_V1.2 from rat, human, and mouse samples.

Anti-SCN2A (Na_V1.2)-ATTO Fluor-594 Antibody (#ASC-002-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-SCN2A (Na_V1.2)-ATTO Fluor-594 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 13/02/2024

Applications

Citations

KO validation citations

Immunohistochemical staining of mouse brain sections with #ASC-002. Tested in SCN2A^-/- mice.
Planells-Cases, R. et al. (2000) Biophys. J. 78, 2878.

Specifications

Scientific Background

Specific Control Product

SCN2A/Nav1.2 Blocking Peptide (#BLP-SC002) is the original antigen used for immunization during Anti-SCN2A (Na_V1.2) Antibody (#ASC-002) generation. The blocking peptide binds and ‘blocks’ Anti-SCN2A/Nav1.2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

SCN2A/Nav1.2 Blocking Peptide (#BLP-SC002)

Shipping and Ordering Information

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Anti-SCN2A (Na_V1.2)-ATTO Fluor-594 Antibody