Name: Anti-SCN2A (NaV1.2)-ATTO Fluor-594 Antibody
Brand: Alomone Labs
SKU: ASC-002-AR

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Overview

Cat #: ASC-002-AR

Alternative Name BII, Brain type II Na⁺ channel, Sodium channel protein type 2 subunit alpha

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)ASAESRDFSGAGGIGVFSE, corresponding to amino acid residues 467-485 of rat Na_V1.2 (Accession P04775). Intracellular loop between domains I and II.

Accession (Uniprot) Number P04775

Gene ID 24766

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Human, mouse – identical.

RRID AB_2341065.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Label ATTO-594. Maximum absorption 601 nm; maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label is related to the Rhodamine dyes and can be used with filters used to detect Texas Red and Alexa-594.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #ASC-002), and immunohistochemistry (labeled antibody).

Applications: if, ih

May also work in: ic*

Immunohistochemistry

Rat hippocampus.
Expression of Na_V1.2 in rat hippocampus
Immunohistochemical staining of rat hippocampus using Anti-SCN2A (Na_V1.2)-ATTO Fluor-594 Antibody (#ASC-002-AR). A. Staining of Na_V1.2 (red) appears in the stratum radiatum (CA3-SR) apical dendrites (arrow) of pyramidal neurons (Pyr). B. DAPI is used as the counterstain (blue). C. Merge images of A and B.

Scientific background

Voltage-gated sodium channels (Na_V) are essential for the generation of action potentials and for cell excitability.¹ Na_V channels are activated in response to depolarization and selectively allow flow of Na⁺ ions. To date, nine Na_V α subunits have been cloned and named Na_V1.1-Na_V1.9.^4-5 The Na_V channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive (Na_V1.1, Na_V1.2, Na_V1.3, Na_V1.4, Na_V1.6 and Na_V1.7) and TTX-resistant (Na_V1.5, Na_V1.8 and Na_V1.9).^2-3

Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain β₁ through β₄ subunits, whereas sodium channels in adult skeletal muscle have only the β₁ subunit.^6,7

Na_V1.2, also known as SCN2A, is primarily expressed in the central nervous system (CNS) and is localized in unmyelinated or premyelinated axons and dendrites. Mutations in the Na_V1.2 channel have been identified in different types of epilepsy.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 13/02/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

SCN2A/Nav1.2 Blocking Peptide (#BLP-SC002) is the original antigen used for immunization during Anti-SCN2A (Na_V1.2) Antibody (#ASC-002) generation. The blocking peptide binds and ‘blocks’ Anti-SCN2A/Nav1.2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

SCN2A/Nav1.2 Blocking Peptide (#BLP-SC002)

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Certificate of Analysis

Anti-SCN2A (Na_V1.2)-ATTO Fluor-594 Antibody