Overview
- Peptide (C)HLEGNHRADGDRFP, corresponding to amino acid residues 511-524 rat NaV1.3 (Accession P08104). Intracellular, loop between domains I and II.
- Western blot analysis of rat newborn brain membranes:1. Anti-SCN3A (NaV1.3) Antibody (#ASC-004), (1:200).
2. Anti-SCN3A (NaV1.3) Antibody, preincubated with SCN3A/Nav1.3 Blocking Peptide (#BLP-SC004). - Mouse membrane lysate (Kim, D.Y. et al. (2011) J. Biol. Chem. 286, 8106.).
- CNahIII-12 cells expressing human NaV1.3 and β1 subunit. (Meadows, L.S. et al. (2002) J. Neurosci. 22, 10669.).
- Expression of NaV1.3 in rat embryo DRGImmunohistochemical staining of rat embryo dorsal root ganglion (DRG) frozen sections using Anti-SCN3A (NaV1.3) Antibody (#ASC-004), (1:100). NaV1.3 is expressed in DRG embryonic cells (arrows). Calibration bar = 50 µm.
- Expression of NaV1.3 in rat DRG primary cellsImmunocytochemical staining of paraformaldehyde-fixed and permeabilized rat dorsal root ganglia (DRG) primary culture. A. DRG cells were stained using Anti-SCN3A (NaV1.3) Antibody (#ASC-004), (1:200) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody. B. Nuclear staining of cells using the cell-permeable dye Hoechst 33342. C. Merged image of panels A and B.
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Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.9.4-5 The NaV channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9).2-3
Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas sodium channels in adult skeletal muscle have only the β1 subunit.6,7
NaV1.3, also known as SCN3A, is highly expressed in embryonic sensory neurons and CNS, but its level dramatically decreases in adult rodents.8 Up-regulation of NaV1.3 channel expression was described in injured neurons and injured spinal cord.9-11
Application key:
Species reactivity key:
Anti-SCN3A (NaV1.3) Antibody (#ASC-004) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, and immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize NaV1.3 from rat, human, and mouse samples.
Applications
Citations
- Expression of NaV1.6 increases in rat mECs during epileptogenesis.A. Immunohistochemical of rat mECs using Anti-NaV1.6 (SCN8A) Antibody (#ASC-009). NaV1.6 staining is detected in AIS and increases in post-SE tissue. The channel co-localizes with Ankryn-G, a marker of AIS. B. Ratio of post-SE and control tissues shows that NaV1.6 expression increases by 46%. C. Somatal expression for NaV1.6 and NaV1.2 (using Anti-SCN2A (NaV1.2) Antibody (#ASC-002)). D. Normalized expression of the channel expression shows that both NaV1.2 and NaV1.6 expression increases in the soma of post-SE tissues. NaV1.1 and NaV1.3 expression, detected using Anti-SCN1A (NaV1.1) Antibody (#ASC-001) and Anti-SCN3A (NaV1.3) Antibody (#ASC-004), respectively, does not change during epileptogenesis.
Adapted from Hargus, N.J. et al. (2013) with permission of the American Physiological Society.
- Human astrocytoma lysate.
Guan, G. et al. (2018) Neurosci. Lett. 674, 148. - Mouse brain lysate.
Lamar, T. et al. (2017) Neurobiol. Dis. 102, 38. - Rat brain neurolemma lysate.
Murenzi, E. et al. (2017) Neurotoxicology 60, 260. - Rat trigeminal ganglion lysate.
Yang, K.Y. et al. (2016) J. Dent. Res. 95, 1183. - Rat pituitary (GH3) cell lysate.
Baroni, D. et al. (2014) Biol. Cell 106, 13. - Rat DRG lysate (1:200).
Cheng, K.I. et al. (2014) Eur. J. Pain 18, 162. - Rat DRG lysate (1:200).
Shen, K.F. et al. (2013) Exp. Neurol. 247, 466. - Mouse brain.
Verret, L. et al. (2012) Cell 149, 708. - Mouse brain membrane lysate.
Kim, D.Y. et al. (2011) J. Biol. Chem. 286, 8106.
- CNahIII-12 cells expressing human NaV1.3 and β1 subunit.
Meadows, L.S. et al. (2002) J. Neurosci. 22, 10669.
- Human brain sections.
Guan, G. et al. (2018) Neurosci. Lett. 674, 148. - Rat lumbar spinal cord sections.
Wolff, M. et al. (2016) Neurosci. Res. 109, 16. - Rat DRGs.
Cheng, K.I. et al. (2014) Eur. J. Pain 18, 162. - Rat brain sections (1:250).
Hargus, N.J. et al. (2013) J. Neurophysiol. 110, 1144. - Rat brain sections (1:200).
Lindia, J.A. and Abbadie, C. (2003) Brain Res. 960, 132.
- Mouse ventricular myocytes.
Koleske, M. et al. (2018) J. Gen. Physiol. 150, 991. - Rat DRGs (1:200).
Shen, K.F. et al. (2013) Exp. Neurol. 247, 466. - Mouse LVA myocytes (1:100).
Ednie, A.R. et al. (2013) J. Mol. Cell. Cardiol. 59, 117.