Name: Anti-SCN3A (NaV1.3) Antibody
Brand: Alomone Labs
SKU: ASC-004

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Overview

Cat #: ASC-004

Alternative Name BIII, Brain type III Na⁺ channel, Sodium channel protein type 3 subunit alpha

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)HLEGNHRADGDRFP, corresponding to amino acid residues 511-524 rat Na_V1.3 (Accession P08104). Intracellular, loop between domains I and II.

Accession (Uniprot) Number P08104

Gene ID 497770

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - 12/14 amino acid residues identical.

RRID AB_2040007.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN_3.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ic, if, ih, ip, wb

May also work in: ifc*

Western blot

Rat newborn brain membranes (1:200). Addition of 0.1-0.5% Tween-20 to the primary antibody solution is recommended. Mouse membrane lysate (see Kim, D.Y. et al. (2011) J. Biol. Chem. 286, 8106.).
Western blot analysis of rat newborn brain membranes:
1. Anti-SCN3A (Na_V1.3) Antibody (#ASC-004), (1:200).
2. Anti-SCN3A (Na_V1.3) Antibody, preincubated with SCN3A/Nav1.3 Blocking Peptide (#BLP-SC004).

Immunoprecipitation

CNahIII-12 cells expressing human Na_V1.3 and β1 subunit. (Meadows, L.S. et al. (2002) J. Neurosci. 22, 10669.).

Immunohistochemistry

Rat embryo DRG frozen section.

Immunocytochemistry

Rat DRG primary culture (fixed and permeabilized) (1:200).

Scientific background

Voltage-gated sodium channels (Na_V) are essential for the generation of action potentials and for cell excitability.¹ Na_V channels are activated in response to depolarization and selectively allow flow of Na⁺ ions. To date, nine Na_V α subunits have been cloned and named Na_V1.1-Na_V1.9.^4-5The Na_V channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive (Na_V1.1, Na_V1.2, Na_V1.3, Na_V1.4, Na_V1.6 and Na_V1.7) and TTX-resistant (Na_V1.5, Na_V1.8 and Na_V1.9).^2-3

Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain β₁ through β₄ subunits, whereas sodium channels in adult skeletal muscle have only the β₁ subunit.^6,7

Na_V1.3, also known as SCN3A, is highly expressed in embryonic sensory neurons and CNS, but its level dramatically decreases in adult rodents.⁸ Up-regulation of Na_V1.3 channel expression was described in injured neurons and injured spinal cord.^9-11

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Image & Title:

Na_V1.1 Levels Decrease in PV Cells of hAPP Mice and in AD Brains.A and B. Western blot analysis of parietal cortex from mice and inferior parietal cortex from humans using Anti-SCN1A (Na_V1.1) Antibody (#ASC-001), Anti-SCN2A (Na_V1.2) Antibody (#ASC-002), Anti-SCN3A (Na_V1.3) Antibody (#ASC-004) and Anti-Na_V1.6 (SCN8A) Antibody (#ASC-009). The graphs represent the quantitation of each western blot.Adapted from Verret, L. et al. (2012) with permission of Elsevier.

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

SCN3A/Nav1.3 Blocking Peptide (#BLP-SC004) is the original antigen used for immunization during Anti-SCN3A (Na_V1.3) Antibody (#ASC-004) generation. The blocking peptide binds and ‘blocks’ Anti-SCN3A/Nav1.3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

SCN3A/Nav1.3 Blocking Peptide (#BLP-SC004)

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Anti-SCN3A (Na_V1.3) Antibody