Name: Guinea pig Anti-NaV1.5 (SCN5A) Antibody
Brand: Alomone Labs
SKU: ASC-005-GP (formerly AGP-008)

Overview

Cat #: ASC-005-GP (formerly AGP-008)

Alternative Name Sodium channel protein type 5 subunit alpha, SKM2

Lyophilized Powder yes

Type: Polyclonal

Host: Guinea pig

Reactivity: h, m, r

Immunogen

Peptide DRLPKSDSEDGPRALNQLSC, corresponding to amino acid residues 493-511 of rat Na_V1.5 (Accession P15389). Intracellular loop between domains I and II.

Accession (Uniprot) Number P15389

Gene ID 25665

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - identical; human - 17/19 amino acid residues identical.

RRID AB_2340960.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Guinea pig total IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.95 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ifc*, ip*

Western blot

Western blot analysis of human-Na_V1.5 transfected HEK-293 cell lysate (lanes 1 and 3) and rat heart membrane protein (lanes 2 and 4):
1,2. Guinea pig Anti-Na_V1.5 (SCN5A) Antibody (#ASC-005-GP), (1:200).
3,4. Guinea pig Anti-Na_V1.5 (SCN5A) Antibody, preincubated with Nav1.5/SCN5A (493-511) Blocking Peptide (#BLP-SC005).

Immunohistochemistry

Expression of Na_V1.5 in rat heart
Immunohistochemical staining of rat heart frozen sections using Guinea pig Anti-Na_V1.5 (SCN5A) Antibody (#ASC-005-GP). Na_V1.5 staining (red) appears in cardiomyocytes and in intercalated discs (arrows). Nuclei are stained using DAPI (blue).
Human heart sections (1:200) (Cheng, J. et al. (2017) Sci. Rep. 7, 42953.).

References

Wu, L. et al. (2002) Neuroreport. 13, 2547.
Liu, C.J. et al. (2003) J. Biol. Chem. 278, 1029.

Scientific background

Voltage-gated Na⁺ channels (Na_V) are responsible for myocardial conduction and maintenance of the cardiac rhythm and are essential for the generation of action potentials and cell excitability¹.

Dysfunction or disregulation of cardiac sodium channels can cause several disorders, including cardiac arrhythmias.

The majority of Na⁺ channels in the mammalian heart are Tetrodotoxin (TTX)-insensitive Na_V1.5².

The putative structure of Na_V1.5 consists of four homologous domain (I-IV), each containing six transmembrane segments (S1-S6). Mutations in the C-terminus of Na_V1.5 were described in connection to Long QT syndrome and Brugada syndrome^1,2. Recent data have demonstrated selective expression of Na_V1.5 in the mouse central nervous system and implicated a role for Na_V1.5 in the physiology of the central nervous system¹.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Guinea pig Anti-Na_V1.5 (SCN5A) Antibody (#ASC-005-GP), raised in guinea pigs, is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Na_V1.5 sodium channel from rat, human and mouse samples.
The antigen used to immunize guinea pigs is the same as Anti-Na_V1.5 (SCN5A) (493-511) Antibody (#ASC-005) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Certificate of Analysis SDS

Image & Title:

Multiplex staining of Na_V1.5 and Vinculin in human heart.Immunohistochemical staining of human ventricle heart sections using Guinea pig Anti-Na_V1.5 (SCN5A) Antibody (#ASC-005-GP) and mouse anti-vinculin antibody. Na_V1.5 staining (green) is detected in intercalated disks and co-localizes with vinculin.Adapted from Cheng, J. et al. (2017) Sci. Rep. 7, 42953. with permission of SPRINGER NATURE.

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Immunohistochemistry citations

Human heart sections (1:200).
Cheng, J. et al. (2017) Sci. Rep. 7, 42953.

Specifications

Scientific Background

Specific Control Product

Nav1.5/SCN5A (493-511) Blocking Peptide (#BLP-SC005) is the original antigen used for immunization during Anti-Na_V1.5 (SCN5A) (493-511) Antibody (#ASC-005) generation. The blocking peptide binds and ‘blocks’ Anti-Nav1.5/SCN5A (493-511) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Nav1.5/SCN5A (493-511) Blocking Peptide (#BLP-SC005)

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.

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Guinea pig Anti-Na_V1.5 (SCN5A) Antibody