Name: Anti-NaV1.5 (SCN5A) (extracellular) Antibody
Brand: Alomone Labs
SKU: ASC-045

Overview

Cat #: ASC-045

Alternative Name Sodium channel protein type 5 subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.5, Long QT Syndrome 3, LQT3

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide CESFNVTGELYWTKVK, corresponding to amino acid residues 1386 - 1401 of mouse SCN5A (Accession Q9JJV9). Extracellular, 11^th loop (3^rd loop of 3^rd repeat).

Accession (Uniprot) Number Q9JJV9

Gene ID 20271

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat – identical, Human – 14 out of 16 residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preadsorption Control 1 µg peptide per 1 µg antibody
Na_V1.5 (SCN5A) (extracellular) Blocking Peptide (#BLP-SC045)

Standard quality control of each lot Western blot analysis.

Applications: ifc, lci, wb

May also work in: ic*, ih*, ip*

Western blot

Western blot analysis of rat heart lysate:
1. Anti-Na_V1.5 (SCN5A) (extracellular) Antibody (#ASC-045), (1:400).
2. Anti-Na_V1.5 (SCN5A) (extracellular) Antibody, preincubated with Na_V1.5 (SCN5A) (extracellular) Blocking Peptide (BLP-SC045).
Western blot analysis of human MDA-MB-231 breast adenocarcinoma cell line lysate (lanes 1 and 3) and human Jurkat T-cell leukemia cell line lysate (lanes 2 and 4):
1-2. Anti-Na_V1.5 (SCN5A) (extracellular) Antibody (#ASC-045), (1:200).
3-4. Anti-Na_V1.5 (SCN5A) (extracellular) Antibody, preincubated with Na_V1.5 (SCN5A) (extracellular) Blocking Peptide (BLP-SC045).

Indirect flow cytometry

Cell surface detection of Nav1.5 by indirect flow cytometry in live intact human Jurkat T-cell leukemia cell line:
^___ Cells.
^___ Cells + goat-anti-rabbit-APC.
^___ Cells + Anti-Na_V1.5 (SCN5A) (extracellular) Antibody (#ASC-045), (5μg) + goat-anti-rabbit-APC.
Cell surface detection of Na_V1.5 by indirect flow cytometry in live intact human MDA-MB-231 breast adenocarcinoma cell line:
^___ Cells.
^___ Cells + goat-anti-rabbit-APC.
^___ Cells + Anti-Na_V1.5 (SCN5A) (extracellular) Antibody (#ASC-045), (5μg) + goat-anti-rabbit-APC.

References

Wu, L. et al. (2002) NeuroReport 13, 2547.
Liu, C.J. et al. (2003) J. Biol. Chem. 278, 1029.

Scientific background

Voltage-gated Na⁺ channels (Na_V) are responsible for myocardial conduction and maintenance of the cardiac rhythm and are essential for the generation of action potentials and cell excitability.¹Dysfunction or disregulation of cardiac sodium channels can cause several disorders, including cardiac arrhythmias.

The majority of Na⁺ channels in the mammalian heart are Tetrodotoxin (TTX)-insensitive Na_V1.5.²

The putative structure of Na_V1.5 consists of four homologous domains (I-IV), each containing six transmembrane segments (S1-S6). Mutations in the C-terminus of Na_V1.5 were described in connection to Long QT syndrome and Brugada syndrome.^1-2 Recent data have demonstrated selective expression of Na_V1.5 in the mouse central nervous system and implicated a role for Na_V1.5 in the physiology of the central nervous system.¹

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-Na_V1.5 (SCN5A) (extracellular) Antibody (#ASC-045) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and flow cytometry applications. It has been designed to recognize Na_V1.5 sodium channel from rat, human, and mouse samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Na_V1.5 (SCN5A) (extracellular) Blocking Peptide (#BLP-SC045) is the original antigen used for immunization during Anti-Na_V1.5 (SCN5A) (extracellular) Antibody (#ASC-045) generation. The blocking peptide binds and ‘blocks’ Anti-Na_V1.5 (SCN5A) (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Na_V1.5 (SCN5A) (extracellular) Blocking Peptide (#BLP-SC045)

Shipping and Ordering Information

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Anti-Na_V1.5 (SCN5A) (extracellular) Antibody