Name: Anti-NaV1.8 (SCN10A) Antibody
Brand: Alomone Labs
SKU: ASC-016

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Overview

Cat #: ASC-016

Alternative Name PN3, SNS, Sodium channel protein type 10 subunit alpha

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

May also work in: h*

Immunogen

Peptide (C)EDEVAAKEGNSPGPQ, corresponding to amino acid residues 1943-1956 of rat Na_V1.8 (Accession Q63554). Intracellular, C-terminus.

Accession (Uniprot) Number Q63554

Gene ID 29571

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - 14/15 amino acid residues identical; human - 7/15 amino acid residues identical.

RRID AB_2040188.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.9 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ic, if, ih, ip, wb

May also work in: ifc*

Western blot

Rat DRG lysates (1:200). Addition of 0.1% Tween-20 to the blocking and to the antibody solution is recommended.
Western blot analysis of rat DRG lysates:
1. Anti-Na_V1.8 (SCN10A) Antibody (#ASC-016), (1:200).
2. Anti-Na_V1.8 (SCN10A) Antibody, preincubated with Nav1.8/SCN10A Blocking Peptide (#BLP-SC016).

Immunoprecipitation

Rat dorsal root ganglia (15 µg) (Tan, Z.Y. et al. (2014) J. Neurosci. 34, 7190.).

Immunohistochemistry

Adult rat DRG frozen sections. Human neuromas (1:100) (Black, J.A. et al. (2008) Ann. Neurol. 64, 644.).

Immunocytochemistry

Rat dorsal root ganglia (DRG) (1:500) (Belkouch, M. et al. (2011) J. Neurosci. 31, 18381.).

Scientific background

Voltage-gated Na⁺ channels (Na_V) are essential for the generation of action potentials and for cell excitability.¹ Na_V channels are activated in response to depolarization and selectively allow flow of Na⁺ ions. To date, nine Na_V α subunits have been cloned and named Na_V1.1-1.9.^2-3 The Na_V channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels.^4-5 Expression of the α subunit isoform is developmentally and tissue specific.

Two TTX-resistant Na_V channels are expressed in dorsal root ganglion (DRG) neurons, Na_V1.8 and Na_V1.9. The Na_V1.8 channel (also called SCN10A, SNS and PN3) is mainly expressed in small-diameter DRG neurons.^4-6 TTX-resistant channels have been suggested to play an important role in nociceptive transmission.

Recently, involvement of Na_V1.8 in multiple sclerosis (MS) was suggested due to up-regulation of both mRNA and protein in Purkinje cells of MS patients and also in animal models.⁶

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Image & Title:

Expression of Na_V1.8 in rat DRG and sciatic nerve.Immunohistochemical staining of rat DRG and sciatic nerve using Anti-Na_V1.8 (SCN10A) Antibody (#ASC-016). A. Na_V1.8 (red) and KIF5B co-localize in DRG and sciatic nerve. B. Na_V1.8 (red) and KIF5B expression increases following inflammation induction.Adapted from Su, Y.Y. et al. (2013) with permission of the Society for Neuroscience.

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

Nav1.8/SCN10A Blocking Peptide (#BLP-SC016) is the original antigen used for immunization during Anti-Na_V1.8 (SCN10A) Antibody (#ASC-016) generation. The blocking peptide binds and ‘blocks’ Anti-Nav1.8/SCN10A primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Nav1.8/SCN10A Blocking Peptide (#BLP-SC016)

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Certificate of Analysis

Anti-Na_V1.8 (SCN10A) Antibody