Name: Anti-NaV1.8 (SCN10A) (extracellular) Antibody
Brand: Alomone Labs
SKU: ASC-028

Overview

Cat #: ASC-028

Alternative Name PN3, SNS, Sodium channel protein type 10 subunit alpha

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

Immunogen

Peptide (C)EDNYLEEKPRVKSVLE, corresponding to amino acid residues 1173-1188 of rat Na_V1.8 (Accession Q62968). 1^st extracellular loop, domain III.

Accession (Uniprot) Number Q62968

Gene ID 29571

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - identical; human – Not recommended for use with human samples.

RRID AB_2341070.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Specificity Not recommended for use with human samples.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.85 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: wb

May also work in: ic*, ifc*, ih*, ip*, lci*

Western blot

Western blot analysis of rat brain (lanes 1 and 3) and rat DRG (lanes 2 and 4) lysates:
1,2. Anti-Na_V1.8 (SCN10A) (extracellular) Antibody (#ASC-028), (1:200).
3,4. Anti-Na_V1.8 (SCN10A) (extracellular) Antibody, preincubated with Nav1.8/SCN10A (extracellular) Blocking Peptide (#BLP-SC028).

References

Wu, L. et al. (2002) NeuroReport 13, 2547.
Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27.
Lai, J. et al. (2003) Curr. Opin. Neurobiol. 13, 291.
Fang, X. et al. (2002) J. Neurosci. 22, 7425.
Fjell, J. et al. (2000) NeuroReport 11, 199.
Renganathan, M. et al. (2003) Brain Res. 959, 235.

Scientific background

Voltage-gated Na⁺ channels (Na_V) are essential for the generation of action potentials and for cell excitability.¹ Na_V channels are activated in response to depolarization and selectively allow flow of Na⁺ ions. To date, nine Na_V α subunits have been cloned and named Na_V1.1-1.9.^2-3 The Na_V channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels.^4-5b Expression of the α subunit isoform is developmentally and tissue specific.

Two TTX-resistant Na_V channels are expressed in dorsal root ganglion (DRG) neurons, Na_V1.8 and Na_V1.9. The Na_V1.8 channel (also called SCN10A, SNS and PN3) is mainly expressed in small-diameter DRG neurons.^4-6 TTX-resistant channels have been suggested to play an important role in nociceptive transmission.

Recently, involvement of Na_V1.8 in multiple sclerosis (MS) was suggested due to up-regulation of both mRNA and protein in Purkinje cells of MS patients and also in animal models.⁶

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat Na_V1.8 channel. Anti-Na_V1.8 (SCN10A) (extracellular) Antibody (#ASC-028) can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting Na_V1.8 in living cells. It has been designed to recognize Na_V1.8 from rat and mouse samples. The antibody will not recognize Na_V1.8 from human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Nav1.8/SCN10A (extracellular) Blocking Peptide (#BLP-SC028) is the original antigen used for immunization during Anti-Na_V1.8 (SCN10A) (extracellular) Antibody (#ASC-028) generation. The blocking peptide binds and ‘blocks’ Anti-Nav1.8/SCN10A (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Nav1.8/SCN10A (extracellular) Blocking Peptide (#BLP-SC028)

Shipping and Ordering Information

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Anti-Na_V1.8 (SCN10A) (extracellular) Antibody