Overview
- Peptide (C)EDNYLEEKPRVKSVLE, corresponding to amino acid residues 1173-1188 of rat NaV1.8 (Accession Q62968). 1st extracellular loop, domain III.
- Rat brain and DRG lysates (1:200- 1:1000).
Western blot analysis of rat brain (lanes 1 and 3) and rat DRG (lanes 2 and 4) lysates:1,2. Anti-NaV1.8 (SCN10A) (extracellular) Antibody (#ASC-028), (1:200).
3,4. Anti-NaV1.8 (SCN10A) (extracellular) Antibody, preincubated with Nav1.8/SCN10A (extracellular) Blocking Peptide (#BLP-SC028).
Voltage-gated Na+ channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-1.9.2-3 The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels.4-5b Expression of the α subunit isoform is developmentally and tissue specific.
Two TTX-resistant NaV channels are expressed in dorsal root ganglion (DRG) neurons, NaV1.8 and NaV1.9. The NaV1.8 channel (also called SCN10A, SNS and PN3) is mainly expressed in small-diameter DRG neurons.4-6 TTX-resistant channels have been suggested to play an important role in nociceptive transmission.
Recently, involvement of NaV1.8 in multiple sclerosis (MS) was suggested due to up-regulation of both mRNA and protein in Purkinje cells of MS patients and also in animal models.6
