Name: Guinea pig Anti-NaV1.8 (SCN10A) Antibody
Brand: Alomone Labs
SKU: ASC-016-GP (formerly AGP-029)

Overview

Cat #: ASC-016-GP (formerly AGP-029)

Alternative Name PN3, SNS, Sodium channel protein type 10 subunit alpha

Lyophilized Powder yes

Type: Polyclonal

Host: Guinea pig

Reactivity: r

May also work in: h*, m*

Immunogen

Peptide (C)EDEVAAKEGNSPGPQ, corresponding to amino acid residues 1943-1956 of rat Na_V1.8 (Accession Q63554). Intracellular, C-terminus.

Accession (Uniprot) Number Q63554

Gene ID 29571

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - 14/15 amino acid residues identical; human - 7/15 amino acid residues identical.

RRID AB_2340965.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Guinea pig total IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ifc*, ip*

Western blot

Western blot analysis of rat DRG lysate:
1. Guinea pig Anti-Na_V1.8 (SCN10A) Antibody (#ASC-016-GP), (1:200).
2. Guinea pig Anti-Na_V1.8 (SCN10A) Antibody, preincubated with Nav1.8/SCN10A Blocking Peptide (#BLP-SC016).

Immunohistochemistry

Expression of Na_V1.8 in rat DRG
Immunohistochemical staining of rat dorsal root ganglion (DRG) using Guinea pig Anti-Na_V1.8 (SCN10A) Antibody (#ASC-016-GP). A. Na_V1.8 staining (green) appears in the cell bodies of the DRG neurons. B. Nuclear staining using DAPI as the counter stain (blue). C. Merged image of A and B.

References

Wu, L. et al. (2002) NeuroReport 13, 2547.
Baker, M.D. et al. (2001) Trends Pharmacol. Sci. 22, 27.
Lai, J. et al. (2003) Curr. Opin. Neurobiol. 13, 291.
Fang, X. et al. (2002) J. Neurosci. 22, 7425.
Fjell, J. et al. (2000) NeuroReport 11, 199.
Renganathan, M. et al. (2003) Brain Res. 959, 235.

Scientific background

Voltage-gated Na⁺ channels (Na_V) are essential for the generation of action potentials and for cell excitability¹. Na_V channels are activated in response to depolarization and selectively allow flow of Na⁺ ions. To date, nine Na_V α subunits have been cloned and named Na_V1.1-1.9^2,3. The Na_V channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels^4,5. The expression of the α subunit isoform is developmentally regulated and tissue specific.

Two TTX-resistant Na_V channels are expressed in dorsal root ganglion (DRG) neurons, Na_V1.8 and Na_V1.9. The Na_V1.8 channel (also called SCN10A, SNS and PN3) is mainly expressed in small-diameter DRG neurons^4-6. TTX-resistant channels have been suggested to play an important role in nociceptive transmission.

Recently, involvement of Na_V1.8 in multiple sclerosis (MS) was suggested due to up-regulation of both mRNA and protein in Purkinje cells of MS patients and also in animal models⁶.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat Na_V1.8 channel. Guinea pig Anti-Na_V1.8 (SCN10A) Antibody (#ASC-016-GP), raised in guinea pigs can be used in western blot and immunohistochemistry applications. It has been designed to recognize Na_V1.8 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-Na_V1.8 (SCN10A) Antibody (#ASC-016) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Nav1.8/SCN10A Blocking Peptide (#BLP-SC016) is the original antigen used for immunization during Anti-Na_V1.8 (SCN10A) Antibody (#ASC-016) generation. The blocking peptide binds and ‘blocks’ Anti-Nav1.8/SCN10A primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Nav1.8/SCN10A Blocking Peptide (#BLP-SC016)

Shipping and Ordering Information

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Guinea pig Anti-Na_V1.8 (SCN10A) Antibody