Overview
- Peptide CNGDLSSLDVAKVKVHND, corresponding to amino acid residues 1748-1765 of rat NaV1.9 (Accession O88457). Intracellular, C-terminus.
- Rat DRG lysates (1:200).
Western blot analysis of rat DRG lysates:1. Anti-SCN11A (NaV1.9) Antibody (#ASC-017), (1:200).
2. Anti-SCN11A (NaV1.9) Antibody, preincubated with SCN11A/Nav1.9 Blocking Peptide (#BLP-SC017).
- Rat embryo DRG formalin frozen sections. Human trigeminal ganglia and dental pulp (1:100) (see Wells, J.E. et al. (2007) J. Endodontics 33, 1172.).
- Rat dorsal root ganglion (DRG) primary culture (1:500).
Voltage-gated Na+ channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-1.9.2-3 Expression of the α subunit is developmentally and tissue specific.
The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels.4-5 TTX-resistant channels have been suggested to play an important role in nociceptive transmission. Two TTX-resistant NaV channels are expressed in dorsal root ganglion (DRG) neurons, NaV1.8 and NaV1.9. The NaV1.9 channel (also called SCN11A, NAN or SNS2) is preferentially expressed in small-diameter DRG neurons.4-5
Recently, it was shown that BDNF activates NaV1.9 channels in the hippocampus suggesting that its expression is not restricted to DRG alone.6
