Name: Guinea pig Anti-SCN11A (NaV1.9) Antibody
Brand: Alomone Labs
SKU: ASC-017-GP

Overview

Cat #: ASC-017-GP

Alternative Name PN5, SNS2, NAN, Sodium channel protein type 11 subunit alpha

Lyophilized Powder yes

Type: Polyclonal

Host: Guinea pig

Reactivity: r

May also work in: h*, m*

Immunogen

Peptide CNGDLSSLDVAKVKVHND, corresponding to amino acid residues 1748-1765 of rat Na_V1.9 (Accession O88457). Intracellular, C-terminus.

Accession (Uniprot) Number O88457

Gene ID 29701

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - 15/18 amino acid residues identical; human - 14/18 amino acid residues identical.

RRID AB_2340966.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Guinea pig total IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ifc*, ip*

Western blot

Western blot analysis of rat brain membrane:
1. Guinea pig Anti-SCN11A (Na_V1.9) Antibody (#ASC-017-GP), (1:200).
2. Guinea pig Anti-SCN11A (Na_V1.9) Antibody, preincubated with SCN11A/Nav1.9 Blocking Peptide (#BLP-SC017).

Immunohistochemistry

Expression of Na_V1.9 in rat DRG
Immunohistochemical staining of Na_V1.9 in rat dorsal root ganglion (DRG) using Guinea pig Anti-SCN11A (Na_V1.9) Antibody (#ASC-017-GP). A. Na_V1.9 staining (green) appears in the cell bodies of the DRG neurons. B. Nuclear staining using DAPI as the counter stain (blue). C. Merged image of A and B.

References

Wu, L. et al. (2002) NeuroReport 13, 2547.
Baker, M.D. et al. (2001) Trends Pharmacol. Sci. 22, 27.
Lai, J. et al. (2003) Curr. Opin. Neurobiol. 13, 291.
Fang, X. et al. (2002) J. Neurosci. 22, 7425.
Fjell, J. et al. (2000) NeuroReport 11, 199.
Blum, R. et al. (2002) Nature 419, 687.

Scientific background

Voltage-gated Na⁺ channels (Na_V) are essential for the generation of action potentials and for cell excitability¹. Na_V channels are activated in response to depolarization and selectively allow the flow of Na⁺ ions. To date, nine Na_V α subunits have been cloned and named Na_V1.1-1.9^2,3. Their expression is developmentally and tissue specific.

Na_V channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels^4,5. TTX-resistant channels have been suggested to play an important role in nociceptive transmission. Two TTX-resistant Na_V channels are expressed in dorsal root ganglion (DRG) neurons, Na_V1.8 and Na_V1.9. The Na_V1.9 channel (also called SCN11A, NAN or SNS2) is preferentially expressed in small-diameter DRG neurons^4,5.

Recently, it has been shown that BDNF activated Na_V1.9 channels in the hippocampus suggesting that its expression is not restricted to DRG alone⁶.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat protein. Guinea pig Anti-SCN11A (Na_V1.9) Antibody (#ASC-017-GP, formerly AGP-030), raised in guinea pigs, can be used in western blot and immunohistochemical applications, and has been designed to recognize Na_V1.9 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-SCN11A (Na_V1.9) Antibody (#ASC-017) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 21/11/2024

Applications

Citations

Immunohistochemistry citations

Rat brain sections (5 µg/ml).
Kurowski, P. et al. (2015) Neuroscience 303, 474.

Specifications

Scientific Background

Specific Control Product

SCN11A/Nav1.9 Blocking Peptide (#BLP-SC017) is the original antigen used for immunization during Anti-SCN11A (Na_V1.9) Antibody (#ASC-017) generation. The blocking peptide binds and ‘blocks’ Anti-SCN11A/Nav1.9 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

SCN11A/Nav1.9 Blocking Peptide (#BLP-SC017)

Shipping and Ordering Information

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Products are lyophilized and ship at room temperature - FREE OF CHARGE.

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Guinea pig Anti-SCN11A (NaV1.9) Antibody