Name: Guinea pig Anti-SCN11A (NaV1.9) Antibody
Brand: Alomone Labs
SKU: ASC-017-GP

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Overview

Cat #: ASC-017-GP

Alternative Name PN5, SNS2, NAN, Sodium channel protein type 11 subunit alpha

Lyophilized Powder yes

Type: Polyclonal

Host: Guinea pig

Reactivity: r

May also work in: h*, m*

Immunogen

Peptide CNGDLSSLDVAKVKVHND, corresponding to amino acid residues 1748-1765 of rat Na_V1.9 (Accession O88457). Intracellular, C-terminus.

Accession (Uniprot) Number O88457

Gene ID 29701

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - 15/18 amino acid residues identical; human - 14/18 amino acid residues identical.

RRID AB_2340966.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Guinea pig total IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ifc*, ip*

Western blot

Rat brain membrane (1:200-1:1000).
Western blot analysis of rat brain membrane:
1. Guinea pig Anti-SCN11A (Na_V1.9) Antibody (#ASC-017-GP), (1:200).
2. Guinea pig Anti-SCN11A (Na_V1.9) Antibody, preincubated with SCN11A/Nav1.9 Blocking Peptide (#BLP-SC017).
Following a broad screen of secondary antibodies, the following was used for this application: #106-035-006 (Jackson ImmunoResearch).

Immunohistochemistry

Rat dorsal root ganglion (DRG). Following a broad screen of secondary antibodies, the following was used for this application: #106-485-006 (Jackson ImmunoResearch).

Scientific background

Voltage-gated Na⁺ channels (Na_V) are essential for the generation of action potentials and for cell excitability¹. Na_V channels are activated in response to depolarization and selectively allow the flow of Na⁺ ions. To date, nine Na_V α subunits have been cloned and named Na_V1.1-1.9^2,3. Their expression is developmentally and tissue specific.

Na_V channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels^4,5. TTX-resistant channels have been suggested to play an important role in nociceptive transmission. Two TTX-resistant Na_V channels are expressed in dorsal root ganglion (DRG) neurons, Na_V1.8 and Na_V1.9. The Na_V1.9 channel (also called SCN11A, NAN or SNS2) is preferentially expressed in small-diameter DRG neurons^4,5.

Recently, it has been shown that BDNF activated Na_V1.9 channels in the hippocampus suggesting that its expression is not restricted to DRG alone⁶.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 21/11/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

SCN11A/Nav1.9 Blocking Peptide (#BLP-SC017) is the original antigen used for immunization during Anti-SCN11A (Na_V1.9) Antibody (#ASC-017) generation. The blocking peptide binds and ‘blocks’ Anti-SCN11A/Nav1.9 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

SCN11A/Nav1.9 Blocking Peptide (#BLP-SC017)

Shipping and Ordering Information

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Certificate of Analysis

Guinea pig Anti-SCN11A (NaV1.9) Antibody