Name: Anti-SCN4B (NaVβ4) (extracellular) Antibody
Brand: Alomone Labs
SKU: ASC-044

Overview

Cat #: ASC-044

Alternative Name Voltage-gated sodium channel subunit beta-4, LQT10

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)KNDKSDPKVRVKDD, corresponding to amino acid residues 85-98 of rat β₄ subunit of voltage-gated Na⁺ channels (Accession Q7M730). Extracellular, N-terminus.

Accession (Uniprot) Number Q7M730

Gene ID 315611

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - identical; human - 11/14 amino acid residues identical.

RRID AB_2341072.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.85 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ic, if, ifc, ih, lci, wb

Western blot

Western blot analysis of rat brain (lanes 1 and 5), rat cortex (lane 2 and 6), mouse brain (lanes 3 and 7) and SH-SY5Y (lanes 4 and 8) lysates:
1-4. Anti-SCN4B (Na_Vβ₄) (extracellular) Antibody (#ASC-044), (1:800).
5-8. Anti-SCN4B (Na_Vβ₄) (extracellular) Antibody, preincubated with SCN4B/Navβ4 (extracellular) Blocking Peptide (#BLP-SC044).

Immunohistochemistry

Expression of Na_Vβ4 in rat DRG
Immunohistochemical staining of adult rat dorsal root ganglion (DRG) using Anti-SCN4B (Na_Vβ₄) (extracellular) Antibody (#ASC-044) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. A. Na_Vβ4 labeling (red) appears in the cell bodies of the DRG neurons. B. Nuclear staining using DAPI as the counterstain (blue). C. Merged image of A and B.

Indirect flow cytometry

Cell surface detection of Na_Vβ4 by indirect flow cytometry in live intact human Jurkat T-cell leukemia cell line:
^___ Cells.
^___ Cells + goat-anti-rabbit-APC.
^___ Cells + Anti-SCN4B (Na_Vβ₄) (extracellular) Antibody (#ASC-044), (2.5μg) + goat-anti-rabbit-APC.

Live cell imaging / Immunocytochemistry

Expression of Na_Vβ4 in rat PC12 cells
Cell surface detection of Na_Vβ4 in intact living rat pheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-SCN4B (Na_Vβ₄) (extracellular) Antibody (#ASC-044), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells.

References

Wu, L. et al. (2002) NeuroReport 13, 2547.
Fang, X. et al. (2002) J. Neurosci. 22, 7425.
Fjell, J. et al. (2000) NeuroReport 11, 199.
Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27.
Lai, J. et al. (2003) Curr. Opin. Neurobiol. 13, 291.
Isom, L.L. (2001) Neuroscientist 7, 42.
Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 575.
Medeiros-Domingo, A. et al. (2007) Circulation 116, 134.
Oyama, F. et al. (2006) J. Neurochem. 98, 518.

Scientific background

Voltage-gated sodium channels (Na_V) are essential for the generation of action potentials and for cell excitability¹. Na_V channels are activated in response to depolarization and selectively allow the flow of Na⁺ ions. To date, nine Na_V α subunits have been cloned and named Na_V1.1-Na_V1.9^4,5. Na_V channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive (Na_V1.1, Na_V1.2, Na_V1.3, Na_V1.4, Na_V1.6 and Na_V1.7) and TTX-resistant (Na_V1.5, Na_V1.8 and Na_V1.9)^2,3.

Mammalian Na⁺ channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Na⁺ channels in the adult central nervous system and heart contain β₁ through β₄ subunits, whereas Na⁺ channels in adult skeletal muscle have only the β₁ subunit^6,7.

Na_Vβ4 has been associated with Long QT syndrome⁸ and with Huntington's disease, showing a distinct down regulation of Na_Vβ4 in the presymptomatic stage of HD mice, whereas other voltage-gated ion channel subunits were later decreased⁹.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-SCN4B (Na_Vβ₄) (extracellular) Antibody (#ASC-044) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting Na_Vβ4 in living cells. It has been designed to recognize Na_Vβ4 from rat, human, and mouse samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Western blot citations

Rat brain neurolemma lysate.
Murenzi, E. et al. (2017) Neurotoxicology 60, 260.

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Order Summary

Anti-SCN4B (Na_Vβ₄) (extracellular) Antibody

Overview

Application key:

Species reactivity key:

Applications

Citations

Specifications

Scientific Background

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