Overview
- Peptide (C)SGQLDAAGFQKIYK, corresponding to amino acid residues 40-53 of rat NCS1 (Accession P62168). Intracellular.
Expression of Neuronal calcium sensor 1 in mouse brainImmunohistochemical staining of mouse brain sections using Anti-NCS1-ATTO Fluor-594 Antibody (#ACS-001-AR). A. In the cerebellum, NCS1 immunostaining (red) appears in the Purkinje cell layer (P), (arrows). B. In the hippocampus NCS1 (red) appears in CA3 pyramidal layer (arrows). Nuclei are stained using DAPI (blue).
- Pandalaneni, S. et al. (2015) J. Biol. Chem. 290, 18744.
- Bellucci, L. et al. (2013) PLoS One. 8, 74383.
- Heidarsson, P.O. et al. (2013) Structure. 21, 1812.
Neuronal calcium sensor-1 (NCS-1) is a member of the neuronal calcium sensor family of EF-hand Ca2+-binding proteins that triggers different biological processes to regulate signal transduction in neurons and photoreceptor cells. This family of proteins includes 15 members and is primarily expressed in neurons.
The protein interacts with the G-protein coupled receptor (GPCR) Dopamine D2 receptor (D2R), and the cognate kinases GRK1 and GRK2. NCS-1 responds to changes in intracellular Ca2+ concentration and is involved in many important physiological functions including the regulation of neurotransmitter release, synaptic plasticity, learning and memory, neurite growth, and neuronal survival1,2.
NCS-1 structure is characterized by 4 helix-loop-helix motifs, called EF-hand motifs able to bind Ca2+ ions. These domains are separated by a short loop containing a conserved glycine residue. NCS-1 is targeted to the plasma membrane by an N-terminal myristoylation group through the action of N-miristoyltransferase. Opposite to the EF-motifs is a wide hydrophobic crevice (HC). This area has the ability to interact with various physiological targets2,3.
NCS-1 dysfunctions are linked to mental conditions, such as bipolar disorder, schizophrenia and autism2.
Anti-NCS1 Antibody (#ACS-001) is a highly specific antibody directed against an epitope of the rat Neuronal calcium sensor 1. The antibody can be used in western blot analysis. It has been designed to recognize NCS1 from rat, mouse, and human samples.
Anti-NCS1-ATTO Fluor-594 Antibody (#ACS-001-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-NCS1-ATTO Fluor-594 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.
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