Name: Anti-Nectin-4 (extracellular) Antibody
Brand: Alomone Labs
SKU: ANR-054

Overview

Cat #: ANR-054

Alternative Name Nectin Cell Adhesion Molecule 4, Poliovirus Receptor-Related Protein 4, PVRL4, NECTIN4

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)KYGLHVNPAYEDR, corresponding to amino acid residues 84 - 96 of mouse Nectin-4 (Accession Q8R007).
Extracellular, N-terminus.

Accession (Uniprot) Number Q8R007

Gene ID 71740

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat – 12 out of 13 amino acid residues identical.
Human – 11 out of 13 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preadsorption Control 1 µg peptide per 1 µg antibody

Standard quality control of each lot Western blot analysis

Applications: ifc, wb

May also work in: ic*, ih*, ip*

Western blot

Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat lung lysate (lanes 2 and 4):
1-2. Anti-Nectin-4 (extracellular) Antibody (#ANR-054), (1:200).
3-4. Anti-Nectin-4 (extracellular) Antibody, preincubated with Nectin-4 (extracellular) Blocking Peptide (BLP-NR054).
Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and human MCF-7 breast adenocarcinoma cell line lysate (lanes 2 and 4):
1-2. Anti-Nectin-4 (extracellular) Antibody (#ANR-054), (1:200).
3-4. Anti-Nectin-4 (extracellular) Antibody, preincubated with Nectin-4 (extracellular) Blocking Peptide (BLP-NR054).

Indirect flow cytometry

Cell surface detection of Nectin-4 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:
^___ Cells.
^___ Cells + goat-anti-rabbit-FITC.
^___ Cells + Anti-Nectin-4 (extracellular) Antibody (#ANR-054), (2.5μg) + goat-anti-rabbit-FITC.

References

Challita-Eidet al (2016), Cancer Res, 76, 3003.
Miyoshi and Takai (2007), Am J Nephrol, 27, 590.
Reymond et al (2001), J Biol Chem, 276, 43205.
Nishiwada et al (2015), Journal of Experimental & Clinical Cancer Research, 34, 30.

Scientific background

Nectin Cell Adhesion Molecule 4 (nectin-4), also known as Poliovirus Receptor-Related Protein 4 (PVRL4), is a Ca^2 + -independent type 1 transmembrane protein that is a member of the immunoglobulin-like adhesion molecules protein family ¹.

All nectins (Nectin-1, Nectin-2, Nectin-3, and Nectin-4) share common structure features: an extracellular region of three Ig-like domains, a transmembrane region, and a cytoplasmic tail region ².

Nectin-4 interacts with afadin, a protein that has a role in cell-cell adhesion, and with nectin-1, but not with nectin-2 or 3 ³.

Nectin-4 was found to be overexpressed in several human cancers, including lung, ovarian, pancreatic, and breast cancer. It was also demonstrated that a soluble form of Nectin-4 is a potential diagnostic marker for several cancers and that knockdown of Nectin-4 inhibits tumor growth and reduces cell-cell contact in certain cancer types ⁴.

Recently, an antibody-drug conjugate designed for the treatment of cancer expressing Nectin-4 was approved for use in bladder cancer patients.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-Nectin-4 (extracellular) Antibody (#ANR-054) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and flow cytometry applications. It has been designed to recognize Nectin-4 from mouse, rat and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Nectin-4 (extracellular) Blocking Peptide (#BLP-NR054) is the original antigen used for immunization during Anti-Nectin-4 (extracellular) Antibody (#ANR-054) generation. The blocking peptide binds and ‘blocks’ Anti-Nectin-4 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Nectin-4 (extracellular) Blocking Peptide (#BLP-NR054)

Shipping and Ordering Information

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Anti-Nectin-4 (extracellular) Antibody