Name: Anti-Nectin-4 (extracellular) Antibody
Brand: Alomone Labs
SKU: ANR-054

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Overview

Cat #: ANR-054

Alternative Name Nectin Cell Adhesion Molecule 4, Poliovirus Receptor-Related Protein 4, PVRL4, NECTIN4

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)KYGLHVNPAYEDR, corresponding to amino acid residues 84 - 96 of mouse Nectin-4 (Accession Q8R007).
Extracellular, N-terminus.

Accession (Uniprot) Number Q8R007

Gene ID 71740

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat – 12 out of 13 amino acid residues identical.
Human – 11 out of 13 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preadsorption Control 1 µg peptide per 1 µg antibody

Standard quality control of each lot Western blot analysis

Applications: ifc, wb

May also work in: ic*, ih*, ip*

Western blot

Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat lung lysate (lanes 2 and 4):
1-2. Anti-Nectin-4 (extracellular) Antibody (#ANR-054), (1:200).
3-4. Anti-Nectin-4 (extracellular) Antibody, preincubated with Nectin-4 (extracellular) Blocking Peptide (BLP-NR054).
Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and human MCF-7 breast adenocarcinoma cell line lysate (lanes 2 and 4):
1-2. Anti-Nectin-4 (extracellular) Antibody (#ANR-054), (1:200).
3-4. Anti-Nectin-4 (extracellular) Antibody, preincubated with Nectin-4 (extracellular) Blocking Peptide (BLP-NR054).

Scientific background

Nectin Cell Adhesion Molecule 4 (nectin-4), also known as Poliovirus Receptor-Related Protein 4 (PVRL4), is a Ca^2 + -independent type 1 transmembrane protein that is a member of the immunoglobulin-like adhesion molecules protein family ¹.

All nectins (Nectin-1, Nectin-2, Nectin-3, and Nectin-4) share common structure features: an extracellular region of three Ig-like domains, a transmembrane region, and a cytoplasmic tail region ².

Nectin-4 interacts with afadin, a protein that has a role in cell-cell adhesion, and with nectin-1, but not with nectin-2 or 3 ³.

Nectin-4 was found to be overexpressed in several human cancers, including lung, ovarian, pancreatic, and breast cancer. It was also demonstrated that a soluble form of Nectin-4 is a potential diagnostic marker for several cancers and that knockdown of Nectin-4 inhibits tumor growth and reduces cell-cell contact in certain cancer types ⁴.

Recently, an antibody-drug conjugate designed for the treatment of cancer expressing Nectin-4 was approved for use in bladder cancer patients.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

Nectin-4 (extracellular) Blocking Peptide (#BLP-NR054) is the original antigen used for immunization during Anti-Nectin-4 (extracellular) Antibody (#ANR-054) generation. The blocking peptide binds and ‘blocks’ Anti-Nectin-4 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Nectin-4 (extracellular) Blocking Peptide (#BLP-NR054)

Shipping and Ordering Information

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Certificate of Analysis

Anti-Nectin-4 (extracellular) Antibody