Overview
- Peptide (C)KIRYRKASRKSDVTE, corresponding to amino acid residues 701 - 715 of mouse Neogenin (Accession P97798). Extracellular, N-term.
Cell surface detection of Neogenin by direct flow cytometry in live intact mouse J774 macrophage cells:___ Cells.
___ Cells + Rabbit IgG isotype control-FITC.
___ Cells + Anti-Neogenin (extracellular)-FITC Antibody (#ANR-195-F), (2.5µg).
Cell surface detection of Neogenin by direct flow cytometry in live intact human Jurkat T-cell leukemia cells:___ Cells.
___ Cells + Rabbit IgG isotype control-FITC.
___ Cells + Anti-Neogenin (extracellular)-FITC Antibody (#ANR-195-F), (5µg).
Cell surface detection of Neogenin by direct flow cytometry in live intact human THP-1 monocytic leukemia cells:___ Cells.
___ Cells + Rabbit IgG isotype control-FITC.
___ Cells + Anti-Neogenin (extracellular)-FITC Antibody (#ANR-195-F), (2.5µg).
Neogenin also known as NEO1 is a member of the immunoglobulin (Ig) superfamily. It has been reported to be involved in diverse physiology and pathology functions, including cell proliferation, differentiation and migration1. Neogenin was originally isolated from embryonic chicken cerebellum and reported to be homologous to the axon guidance receptor deleted in colorectal cancer (DCC)2.
Neogenin consists of 4 N-terminal Ig-like domains, followed by 6 fibronectin type III-like domains, a single transmembrane helix, and an intracellular domain. Neogenin is capable to mediate attractive and repulsive axon guidance depending on the ligand identity. Two main ligands of neogenin are Netrin-1 (NET1) and repulsive guidance molecules (RGMs)3.
Interaction of Neogenin with RGMs leads to cytoskeleton rearrangements via Rho GTPases, this results in growth cone collapse. In contrast interaction with Netrin-1 triggers attractive growth cone response3. Recently it was shown that Neogenin plays a role in the maintenance of iron homeostasis via interaction with HJV (members of the RGM family)4.
