Overview
Alternative Name NTN1, MRMV4
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
- Peptide CRKMYNRPHRAPITK, corresponding to amino acid residues 181 - 195 of rat Netrin-1 (Accession Q924Z9). Secreted.
Accession (Uniprot) Number Q924Z9
Gene ID 114523
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – identical.
RRID AB_2876817
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Reconstitution 0.2 ml double distilled water (DDW).
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ip*
Western blot
Western blot analysis of mouse brain lysates (lanes 1 and 3) and rat brain lysates (lanes 2 and 4):1, 2. Anti-Netrin-1 Antibody (#ANR-121), (1:200).
3, 4. Anti-Netrin-1 Antibody, preincubated with Netrin-1 Blocking Peptide (#BLP-NR121).
Western blot analysis of human U-87 MG glioblastoma cell line lysates (lanes 1 and 3) and human PANC-1 pancreas ductal adenocarcinoma cell line lysates (lanes 2 and 4):1, 2. Anti-Netrin-1 Antibody (#ANR-121), (1:200).
3, 4. Anti-Netrin-1 Antibody, preincubated with Netrin-1 Blocking Peptide (#BLP-NR121).
Immunohistochemistry
Expression of Netrin-1 in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Netrin-1 Antibody (#ANR-121), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Netrin-1 immunoreactivity (green) appears in cells (horizontal arrows) in the hilus (H) in the hippocampal dentate gyrus. B. Pre-incubation of the antibody with Netrin-1 Blocking Peptide (BLP-NR121), suppressed staining. Cell nuclei are stained with DAPI (blue). G= granule layer, H = Hilus.
Expression of Netrin-1 in mouse cerebellum.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Netrin-1 Antibody (#ANR-121), (1:300), followed by goat anti-rabbit-AlexaFluor-488. Netrin-1 immunoreactivity (green) appears in Purkinje cell soma (horizontal arrows) and in Purkinje dendrites (vertical arrows). B. Pre-incubation of the antibody with Netrin-1 Blocking Peptide (BLP-NR121), suppressed staining. Cell nuclei are stained with DAPI (blue). G= granule layer, P = Purkinje layer, M = molecular layer
References
- Serafini, T. et al. (1996) Cell 87, 1001.
- Kennedy, T.E. et al. (2006) J. Neurosci. 26, 8866.
- Kennedy, T.E. et al. (1994) Cell 78, 425.
- Boyer, N.P and Gupton, S.L. (2018) Front. Cell. Neurosci. 12, 221.
Scientific background
Netrins are a family comprised of five members: three secreted proteins (netrins 1, 3 and 4), and two glycosylphosphatidylinositol (GPI)-anchored membrane proteins, netrins G1 and G21. Netrin-1 is a diffusible long-range axonal guidance cue expressed in floor plate cells of the central nervous system. It is secreted in order to direct exons to their target1-3. Axon guidance by netrin-1 is implicated in multiple developing brain regions and developing neuronal types. It also plays a role in axon branching, synaptogenesis, cell migration, cell survival, and axon regeneration4.
Lyophilized Powder
Anti-Netrin-1 Antibody (#ANR-121) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize Netrin-1 from human, mouse, and rat samples.
For research purposes only, not for human use
Last Update: 29/10/2024
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