Overview
Alternative Name NTN1, MRMV4
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
- Peptide CRKMYNRPHRAPITK, corresponding to amino acid residues 181 - 195 of rat Netrin-1 (Accession Q924Z9). Secreted.
Accession (Uniprot) Number Q924Z9
Gene ID 114523
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – identical.
RRID AB_2876817
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Reconstitution 0.2 ml double distilled water (DDW).
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ip*
Western blot
- Mouse and rat brain lysates; human U-87 MG glioblastoma and PANC-1 pancreas ductal adenocarcinoma cell lysates (1:200-1:1000).
Western blot analysis of mouse brain lysates (lanes 1 and 3) and rat brain lysates (lanes 2 and 4):1, 2. Anti-Netrin-1 Antibody (#ANR-121), (1:200).
3, 4. Anti-Netrin-1 Antibody, preincubated with Netrin-1 Blocking Peptide (#BLP-NR121).
Scientific background
Netrins are a family comprised of five members: three secreted proteins (netrins 1, 3 and 4), and two glycosylphosphatidylinositol (GPI)-anchored membrane proteins, netrins G1 and G21. Netrin-1 is a diffusible long-range axonal guidance cue expressed in floor plate cells of the central nervous system. It is secreted in order to direct exons to their target1-3. Axon guidance by netrin-1 is implicated in multiple developing brain regions and developing neuronal types. It also plays a role in axon branching, synaptogenesis, cell migration, cell survival, and axon regeneration4.
Lyophilized Powder
For research purposes only, not for human use
Last Update: 29/10/2024
Specifications
Citations
Citations
