Name: Anti-Netrin-3 Antibody
Brand: Alomone Labs
SKU: ANR-123

Overview

Cat #: ANR-123

Alternative Name NTN3, Netrin-2-Like Protein, NTN2L

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide CRPARGSYRISLKK, corresponding to amino acid residues 445 - 458 of mouse Netrin-3 (Accession Q9R1A3). Secreted.

Accession (Uniprot) Number Q9R1A3

Gene ID 18209

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat – identical; Human – 13 out of 14 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preadsorption Control 1 µg peptide per 1 µg antibody

Standard quality control of each lot Western blot analysis.

Applications: ih, wb

May also work in: ic*, ip*, n*

Western blot

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
1-2. Anti-Netrin-3 Antibody (#ANR-123), (1:400).
3-4. Anti-Netrin-3 Antibody, preincubated with Netrin-3 Blocking Peptide (BLP-NR123).
Western blot analysis of human U-87 MG glioma cell line lysates (lanes 1 and 3) and human SH-SY5Y neuroblastoma cell line lysates (lanes 2 and 4):
1-2. Anti-Netrin-3 Antibody (#ANR-123), (1:200).
3-4. Anti-Netrin-3 Antibody, preincubated with Netrin-3 Blocking Peptide (BLP-NR123).

Immunohistochemistry

Expression of Netrin-3 in rat spinal cord.
Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Anti-Netrin-3 Antibody (#ANR-123), (1:1000), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the ventral horn region showed Netrin-3 immunoreactivity (green) in neuronal profiles (arrows). B. Pre-incubation of the antibody with Netrin-3 Blocking Peptide (BLP-NR123), suppressed staining. Cell nuclei are stained with DAPI (blue).

References

Sun. J., et al (2011) Development. 138, 2153-2169.
Rajasekharan, S, et al (2009) Genome. Biol. 10, 239.
Bruikman, C. et al (2019) J. Nucleic Acids 2019, 9.

Scientific background Netrins are a family of highly conserved proteins responsible for axon guidance and cell movement throughout neural development. Netrins can be divided into secreted netrins (netrin 1,3,4 and 5) and membrane-tethered glycophosphatidylinositol (GPI)-linked netrins (netrin G1 and G2)¹.

Secreted netrins carry their function via interaction with several receptors that include the deleted in colorectal (DCC) family, and the uncoordinated-5 (UNC5-A through UNC5-D) family².

Netrin-3 was discovered in 1997 using sequence homology searching of netrin-2. Similarly to other netrins, Netrin-3 plays an important role in the development of the nervous system. Netrin-3 structure consist of a laminin-like domain located on the N-terminal, three epidermal growth factors like repeats (EGF), and a C-terminal netrin-like domain (NTR). Mutations in NTN3, the gene encoding netrin-3 was found to be associated with the development of several carcinomas³.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-Netrin-3 Antibody (#ANR-123) is a highly specific antibody directed against an epitope of the mouse Netrin-3 protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Netrin-3 from rat, mouse and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Netrin-3 Blocking Peptide (#BLP-NR123) is the original antigen used for immunization during Anti-Netrin-3 Antibody (#ANR-123) generation. The blocking peptide binds and ‘blocks’ Anti-Netrin-3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Netrin-3 Blocking Peptide (#BLP-NR123)

Shipping and Ordering Information

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Anti-Netrin-3 Antibody