Name: Anti-NCAM2 (extracellular) Antibody
Brand: Alomone Labs
SKU: ANR-042

Overview

Cat #: ANR-042

Alternative Name Neural cell adhesion molecule 2, NCAM-2, R4B12, Ocam, Rncam, NCAM21, Neural cell adhesion molecule RB-8

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)KEDQWLEKKVQGNKDH, corresponding to amino acid residues 635-650 of mouse NCAM-2 (Accession O35136). Extracellular, N-terminus.

Accession (Uniprot) Number O35136

Gene ID 17968

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat, human – identical.

RRID AB_2756691.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ifc, ih, lci, wb

May also work in: ic*, ip*

Western blot

Rat and mouse brain lysates and human prostate carcinoma (LNCaP) and breast adenocarcinoma (MCF-7) cell lysates (1:200-1:1000).
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
1,2. Anti-NCAM2 (extracellular) Antibody (extracellular) Antibody (#ANR-042), (1:200).
3,4. Anti-NCAM2 (extracellular) Antibody (extracellular) Antibody, preincubated with NCAM2 (extracellular) Blocking Peptide (#BLP-NR042).
Western blot analysis of human LNCaP prostate carcinoma (lanes 5 and 7) and MCF-7 breast adenocarcinoma (lanes 6 and 8) cell lysates:
5,6. Anti-NCAM2 (extracellular) Antibody (extracellular) Antibody (#ANR-042), (1:200).
7,8. Anti-NCAM2 (extracellular) Antibody (extracellular) Antibody, preincubated with NCAM2 (extracellular) Blocking Peptide (#BLP-NR042).

Immunohistochemistry

Mouse brain sections.

Indirect flow cytometry

Cell surface detection of NCAM2 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:
^___ Cells.
^___ Cells + goat-anti-rabbit-APC.
^___ Cells + Anti-NCAM2 (extracellular) Antibody (#ANR-042), (2.5μg) + goat-anti-rabbit-APC.

Scientific background

Neural cell adhesion molecule 2 (NCAM-2), a protein also known as olfactory cell adhesion molecule (OCAM), belongs to the immunoglobulin superfamily of cell adhesion molecules that participate in homophilic trans-interactions. NCAM-2 is highly expressed in the nervous system and participates in numerous cellular events, such as survival, migration, and differentiation, neurite outgrowth, and formation and plasticity of synapses^1,2.

The NCAM-2 structure consists of five immunoglobulin (Ig) domains followed by two fibronectin-type 3 (Fn3) domains and eight potential sites for N-linked glycosylation³.

Overexpression of NCAM-2 is believed to be a contributing factor to Down syndrome. There is also a genetic association between NCAM-2 and Alzheimer's disease, where NCAM-2 is involved in the pathogenic pathway to the senile plaques that concentrate in the Alzheimer's disease brain¹.

During human embryonic development, NCAM-2 is expressed in several tissues, including lung, liver, and kidney with the highest expression in the brain. NCAM-2 expression level rises around postnatal day 21 and remains high during adulthood. Studies conducted on NCAM-2 deficient mice show that the protein is important for the development of the brain and the olfactory system¹.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

NCAM2 (extracellular) Blocking Peptide (#BLP-NR042) is the original antigen used for immunization during Anti-NCAM2 (extracellular) Antibody (#ANR-042) generation. The blocking peptide binds and ‘blocks’ Anti-NCAM2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

NCAM2 (extracellular) Blocking Peptide (#BLP-NR042)

Shipping and Ordering Information

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Certificate of Analysis

Anti-NCAM2 (extracellular) Antibody